Construct: sgRNA BRDN0001145768
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGCAGCTTTCCGCACCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAMKV (79012)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75526
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
49862087 |
+ |
CAMKV |
NNNCAGCTTTCCGCACCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
77615618 |
- |
LINGO1 |
NNNCAGCTTCCCGCACCTGG |
NGG |
2 |
0.1333 |
Tier I |
| 3 |
NC_000008.11 |
8 |
144791993 |
+ |
RPL8 |
NNNCAGCGTTCCGCACCTTG |
NCG |
2 |
0.0786 |
Tier I |
| 4 |
NC_000016.10 |
16 |
26124003 |
+ |
HS3ST4 |
NNNCAGCTTACCGCAACTTG |
NGG |
2 |
0.8571 |
Tier II |
| 5 |
NC_000011.10 |
11 |
96262873 |
+ |
MAML2 |
NNNCAGCTTTCTGCACCTTC |
NGG |
2 |
0.2308 |
Tier II |
| 6 |
NC_000012.12 |
12 |
50786223 |
- |
ATF1 |
NNNCAGCTTTCTGCATCTTG |
NGG |
2 |
0.1657 |
Tier II |
| 7 |
NC_000020.11 |
20 |
42924600 |
- |
PTPRT |
NNNCAGCTTTCTTCACCTTG |
NGG |
2 |
0.1615 |
Tier II |
| 8 |
NC_000015.10 |
15 |
68560774 |
+ |
CORO2B |
NNNCAGCTTTCAGGACCTTG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000001.11 |
1 |
84795780 |
+ |
LOC102724892 |
NNNCAGCATTCAGCACCTTG |
NGG |
2 |
0.5714 |
Tier III |
| 10 |
NC_000015.10 |
15 |
61545997 |
- |
LOC107984782 |
NNNCAGCTTTCAGCACCTTG |
NAG |
2 |
0.1852 |
Tier III |
| 11 |
NC_000015.10 |
15 |
77615618 |
- |
LOC105370906 |
NNNCAGCTTCCCGCACCTGG |
NGG |
2 |
0.1333 |
Tier III |
| 12 |
NC_000008.11 |
8 |
144791993 |
+ |
MIR6850 |
NNNCAGCGTTCCGCACCTTG |
NCG |
2 |
0.0786 |
Tier III |
| 13 |
NC_000020.11 |
20 |
54186439 |
+ |
LOC105372675 |
NNNCAGCTTTCCGGACCTTG |
NAG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
107945579 |
- |
Camkv |
NNNCTGCCTTCCGCACCTTG |
NGG |
2 |
0.3636 |
Tier I |
| 2 |
NC_000078.6 |
12 |
3806232 |
- |
Dnmt3a |
NNNAAGCTTTCCGTACCTTG |
NGG |
2 |
0.6175 |
Tier II |
| 3 |
NC_000075.6 |
9 |
117318157 |
- |
Rbms3 |
NNNCAGCTTTCCTCACCATG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000070.6 |
4 |
98021403 |
+ |
Nfia |
NNNCAGCTTTCCCCACTTTG |
NGG |
2 |
0.1965 |
Tier II |
| 5 |
NC_000070.6 |
4 |
134343354 |
- |
Slc30a2 |
NNNCAGCTTCCCGCCCCTTG |
NGG |
2 |
0.1455 |
Tier II |
| 6 |
NC_000069.6 |
3 |
95064820 |
+ |
Pip5k1a |
NNNCAGCTTTCTGCACCTTG |
NAG |
2 |
0.1396 |
Tier II |
| 7 |
NC_000083.6 |
17 |
28157018 |
+ |
Scube3 |
NNNCAGCTTTCCCCACCTTG |
NAG |
2 |
0.1092 |
Tier II |
| 8 |
NC_000080.6 |
14 |
101542930 |
- |
Tbc1d4 |
NNNCAGCTTTCCACACCTTG |
NTG |
2 |
0.036 |
Tier II |
| 9 |
NC_000078.6 |
12 |
114781366 |
+ |
Igh |
NNNCAGCTTTCTGCACCTTT |
NGG |
2 |
0.3769 |
Tier III |
| 10 |
NC_000075.6 |
9 |
117318157 |
- |
Gm39451 |
NNNCAGCTTTCCTCACCATG |
NGG |
2 |
0.2 |
Tier III |
| 11 |
NC_000070.6 |
4 |
98021403 |
+ |
Gm34218 |
NNNCAGCTTTCCCCACTTTG |
NGG |
2 |
0.1965 |
Tier III |
Other clones with same target sequence:
(none)
