Construct: sgRNA BRDN0001145776
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATAGCGCAAGTGAACTCCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KC1 (26750)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75980
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
213241312 |
+ |
RPS6KC1 |
NNNAGCGCAAGTGAACTCCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
112633135 |
- |
RPH3A |
NNNAGCTCAAGTGAACTCCT |
NGG |
1 |
0.5714 |
Tier II |
| 3 |
NC_000006.12 |
6 |
61649156 |
+ |
KHDRBS2 |
NNNAGTGCAAGGGAACTCCT |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000019.10 |
19 |
41149866 |
- |
LOC124904790 |
NNNAGCTCAAGTGAACTTCT |
NGG |
2 |
0.3673 |
Tier II |
| 5 |
NC_000016.10 |
16 |
27716714 |
- |
KATNIP |
NNNAGCCCAAGGGAACTCCT |
NGG |
2 |
0.3438 |
Tier II |
| 6 |
NC_000001.11 |
1 |
37843003 |
+ |
MTF1 |
NNNAGCACAAGTGAACTCCT |
NAG |
2 |
0.2593 |
Tier II |
| 7 |
NC_000011.10 |
11 |
36089180 |
+ |
LDLRAD3 |
NNNAGGGCAAGTGAACTGCT |
NGG |
2 |
0.0667 |
Tier II |
| 8 |
NC_000010.11 |
10 |
28439418 |
+ |
LINC02652 |
NNNAGCTCAAGTGAACTCCT |
NGG |
1 |
0.5714 |
Tier III |
| 9 |
NC_000016.10 |
16 |
27716714 |
- |
LOC100128079 |
NNNAGCCCAAGGGAACTCCT |
NGG |
2 |
0.3438 |
Tier III |
| 10 |
NC_000009.12 |
9 |
108006723 |
+ |
LOC107987112 |
NNNAGCCCAAGTGGACTCCT |
NGG |
2 |
0.3208 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
190799987 |
- |
Rps6kc1 |
NNNAGCACAAGTGAGCTCCT |
NGG |
2 |
0.65 |
Tier I |
| 2 |
NC_000072.6 |
6 |
5981491 |
- |
Dync1i1 |
NNNAGCACATGTGAACTCCT |
NGG |
2 |
0.8824 |
Tier II |
| 3 |
NC_000086.7 |
X |
169973011 |
- |
Mid1 |
NNNAGCGGAAGTGAACCCCT |
NGG |
2 |
0.4538 |
Tier II |
| 4 |
NC_000069.6 |
3 |
145292780 |
+ |
Col24a1 |
NNNACCGCAAGTGAACTCCA |
NGG |
2 |
0.442 |
Tier II |
| 5 |
NC_000070.6 |
4 |
132693613 |
- |
Eya3 |
NNNAGCGGAAGGGAACTCCT |
NGG |
2 |
0.3214 |
Tier II |
| 6 |
NC_000068.7 |
2 |
153499335 |
- |
Nol4l |
NNNAGCTCAAGTGAACTCCA |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000086.7 |
X |
13797276 |
+ |
Cask |
NNNAGAGCAAGTGAACTCCT |
NAG |
2 |
0.2407 |
Tier II |
| 8 |
NC_000082.6 |
16 |
28887052 |
+ |
Mb21d2 |
NNNAGAGCAAGTGAAGTCCT |
NGG |
2 |
0.1429 |
Tier II |
| 9 |
NC_000075.6 |
9 |
72445150 |
- |
Mns1 |
NNNAGCGCATGTGAAGTCCT |
NGG |
2 |
0.1357 |
Tier II |
| 10 |
NC_000080.6 |
14 |
75457236 |
+ |
Siah3 |
NNNAGCGCAACTGAACTCCC |
NGG |
2 |
0.039 |
Tier II |
| 11 |
NC_000082.6 |
16 |
8588299 |
- |
Abat |
NNNAGCGCACGTGAACTCCT |
NGC |
2 |
0.0074 |
Tier II |
| 12 |
NC_000085.6 |
19 |
55912155 |
+ |
Tcf7l2 |
NNNAGCGCAAGTGAAGTCCT |
NTG |
2 |
0.006 |
Tier II |
| 13 |
NC_000073.6 |
7 |
83538868 |
- |
Gm10610 |
NNNAGCACAAGTGAACTCTT |
NGG |
2 |
0.4615 |
Tier III |
| 14 |
NC_000080.6 |
14 |
53801418 |
- |
Tcra |
NNNAGCTCAAGTGAGCTCCT |
NGG |
2 |
0.3714 |
Tier III |
| 15 |
NC_000068.7 |
2 |
153499335 |
- |
4930404H24Rik |
NNNAGCTCAAGTGAACTCCA |
NGG |
2 |
0.3214 |
Tier III |
| 16 |
NC_000080.6 |
14 |
75457236 |
+ |
Gm41212 |
NNNAGCGCAACTGAACTCCC |
NGG |
2 |
0.039 |
Tier III |
Other clones with same target sequence:
(none)
