Construct: sgRNA BRDN0001145783

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGGTGTCATCAAGTCACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK5 (8550)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76449

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 111871135 - MAPKAPK5 NNNGTGTCATCAAGTCACCT NGG 0 1.0 Tier I
2 NC_000019.10 19 35720974 + KMT2B NNNGTGTCATCAAGACACCC NGG 2 0.0526 Tier I
3 NC_000010.11 10 60096614 - ANK3 NNNGTGTCAAAAAGTCACCT NGG 2 0.6429 Tier II
4 NC_000012.12 12 117629161 - KSR2 NNNGGGTCATCAACTCACCT NGG 2 0.2743 Tier II
5 NC_000016.10 16 81337273 - GAN NNNATGTCATCATGTCACCT NGG 2 0.27 Tier II
6 NC_000006.12 6 143866639 + ZC2HC1B NNNATGTCATCAAGTCACCT NAG 2 0.2333 Tier II
7 NC_000002.12 2 232182002 + DIS3L2 NNNGTTTCATCATGTCACCT NGG 2 0.2 Tier II
8 NC_000012.12 12 14490908 - ATF7IP NNNGGGTCATCAAGTCACCT NAG 2 0.1659 Tier II
9 NC_000003.12 3 175032819 + NAALADL2 NNNGTGTCATCAAGTTACTT NGG 2 0.142 Tier II
10 NC_000002.12 2 213811884 - SPAG16 NNNGTGTCATCCAGCCACCT NGG 2 0.0718 Tier II
11 NC_000023.11 X 6728878 + PUDP NNNGAGTCATCAAGTCTCCT NGG 2 0.0667 Tier II
12 NC_000016.10 16 90005613 + DBNDD1 NNNGTGTCATCAAGTCTCTT NGG 2 0.0615 Tier II
13 NC_000022.11 22 46247654 - CDPF1 NNNGTGTCATCATGTCACCC NGG 2 0.0273 Tier II
14 NC_000010.11 10 23298803 - C10orf67 NNNGTGTCATCAAGCCACCC NGG 2 0.0248 Tier II
15 NC_000010.11 10 43512465 - ZNF487 NNNGGGTCATCAAGTCACCT NGC 2 0.0142 Tier II
16 NC_000001.11 1 77185960 + PIGK NNNGTGTCATCAAGTCACCA NGC 2 0.0125 Tier II
17 NC_000011.10 11 101918813 + CEP126 NNNGTGTCATCAAGTCATCT NGT 2 0.0104 Tier II
18 NC_000018.10 18 3519332 - DLGAP1 NNNGTGTCATCAAGTCACAT NGC 2 0.0095 Tier II
19 NC_000002.12 2 40094667 - SLC8A1-AS1 NNNGCTTCATCAAGTCACCT NGG 2 0.6667 Tier III
20 NC_000009.12 9 7557392 + PPIAP33 NNNATGTCACCAAGTCACCT NGG 2 0.48 Tier III
21 NC_000013.11 13 22429373 + LOC107984599 NNNCTGTCAACAAGTCACCT NGG 2 0.4538 Tier III
22 NC_000005.10 5 41096415 + LOC105374739 NNNGTGTCTTCAGGTCACCT NGG 2 0.3913 Tier III
23 NC_000001.11 1 38148201 + LOC105378654 NNNGGGTCATCAAGTCACCA NGG 2 0.36 Tier III
24 NC_000005.10 5 106915677 - LINC01950 NNNGGGTCATCAAGTCACCA NGG 2 0.36 Tier III
25 NC_000011.10 11 107234437 - LOC105369477 NNNGGGTCATCAAGTCACCA NGG 2 0.36 Tier III
26 NC_000005.10 5 61910837 - LOC105378998 NNNGAGTCATCAAGTCACCA NGG 2 0.2813 Tier III
27 NC_000001.11 1 60246813 + LINC02778 NNNGGGTCATCAAGTCACCT NTG 2 0.0249 Tier III
28 NC_000001.11 1 60246813 + LOC105378761 NNNGGGTCATCAAGTCACCT NTG 2 0.0249 Tier III
29 NC_000006.12 6 22574709 - LOC105374971 NNNGTGTCATCAAGTCACCT NAT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 121534381 + Mapkapk5 NNNGTGTCATCAAATCACCT NGG 1 0.75 Tier I
2 NC_000082.6 16 20608741 + Alg3 NNNGTGGCATCAACTCACCT NGG 2 0.2521 Tier I
3 NC_000077.6 11 4923417 - Thoc5 NNNCTGTCATCAGGTCACCT NGG 2 0.3453 Tier II
4 NC_000076.6 10 80301693 - Apc2 NNNGTGTCAACCAGTCACCT NGG 2 0.2256 Tier II
5 NC_000070.6 4 3731341 - Lyn NNNGTGTCATCTAGACACCT NGG 2 0.193 Tier II
6 NC_000067.6 1 84258232 - Pid1 NNNGTGTCATCATGTCATCT NGG 2 0.1929 Tier II
7 NC_000082.6 16 40850455 - Lsamp NNNGTGGCATGAAGTCACCT NGG 2 0.1471 Tier II
8 NC_000067.6 1 155806608 + Qsox1 NNNGTGTCATCATGTCACTT NGG 2 0.1385 Tier II
9 NC_000073.6 7 144027190 + Shank2 NNNGTGTCATCAGGTGACCT NGG 2 0.1003 Tier II
10 NC_000077.6 11 119738968 - Rptor NNNGTGGCATCAAGTCTCCT NGG 2 0.0784 Tier II
11 NC_000084.6 18 12226808 + Npc1 NNNGTGTCACCAAGTCTCCT NGG 2 0.0711 Tier II
12 NC_000078.6 12 28752195 - Eipr1 NNNGTGTAATCAAGTCACCT NCG 2 0.0696 Tier II
13 NC_000081.6 15 59572774 + Nsmce2 NNNGTGTCATCAAGTAACCT NGC 2 0.0222 Tier II
14 NC_000083.6 17 64029482 + Fer NNNGTGTCATCAAGTCTCCT NGA 2 0.0093 Tier II
15 NC_000067.6 1 152538510 - Rgl1 NNNGAGTCATCAAGTCACCT NGT 2 0.0081 Tier II
16 NC_000068.7 2 93954249 + Gm33160 NNNGTGTCATCCAGACACCT NGG 2 0.1524 Tier III
17 NC_000073.6 7 71155951 - Gm36071 NNNGTGTCATCAAGTCCCCT NGA 2 0.0123 Tier III
Download CSV

Other clones with same target sequence:

(none)