Construct: sgRNA BRDN0001145787
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCGTGCCCACTCGTCGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK15 (225689)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75618
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
143721015 |
- |
MAPK15 |
NNNGTGCCCACTCGTCGCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
47664691 |
- |
BICRA |
NNNGGGCCCACTCGTCACTG |
NGG |
2 |
0.5973 |
Tier II |
| 3 |
NC_000004.12 |
4 |
2914570 |
- |
ADD1 |
NNNGTGCCCACTCCTCACTG |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000001.11 |
1 |
85495848 |
- |
DDAH1 |
NNNGTTGCCACTCGTCGCTG |
NGG |
2 |
0.3137 |
Tier II |
| 5 |
NC_000001.11 |
1 |
121385622 |
+ |
SRGAP2C |
NNNGTGCCCACTCTTTGCTG |
NGG |
2 |
0.0821 |
Tier II |
| 6 |
NC_000001.11 |
1 |
144894393 |
- |
SRGAP2B |
NNNGTGCCCACTCTTTGCTG |
NGG |
2 |
0.0821 |
Tier II |
| 7 |
NC_000001.11 |
1 |
206404364 |
+ |
SRGAP2 |
NNNGTGCCCACTCTTTGCTG |
NGG |
2 |
0.0821 |
Tier II |
| 8 |
NC_000011.10 |
11 |
14162827 |
- |
SPON1 |
NNNGTGCCCACTCTCCGCTG |
NGG |
2 |
0.0727 |
Tier II |
| 9 |
NC_000012.12 |
12 |
2452582 |
- |
CACNA1C |
NNNGTGCCCACTGGTCCCTG |
NGG |
2 |
0.0321 |
Tier II |
| 10 |
NC_000021.9 |
21 |
25041000 |
- |
LINC01692 |
NNNGTGCCCACTCGTAGCTG |
NCG |
2 |
0.1071 |
Tier III |
| 11 |
NC_000001.11 |
1 |
121385622 |
+ |
SRGAP2-AS1 |
NNNGTGCCCACTCTTTGCTG |
NGG |
2 |
0.0821 |
Tier III |
| 12 |
NC_000001.11 |
1 |
144066118 |
+ |
SRGAP2D |
NNNGTGCCCACTCTTTGCTG |
NGG |
2 |
0.0821 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
36778757 |
- |
Zap70 |
NNNGTGTCCACTCGTCTCTG |
NGG |
2 |
0.2031 |
Tier I |
| 2 |
NC_000078.6 |
12 |
98901049 |
+ |
Eml5 |
NNNGCGCCCACCCGTCGCTG |
NGG |
2 |
0.9474 |
Tier II |
| 3 |
NC_000068.7 |
2 |
139957916 |
+ |
Tasp1 |
NNNGTCCCCACTCGTCACTG |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000069.6 |
3 |
88115057 |
- |
Iqgap3 |
NNNGTGCCCACTGGTCTCTG |
NGG |
2 |
0.0341 |
Tier II |
Other clones with same target sequence:
(none)
