Construct: sgRNA BRDN0001145789
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATATGGCACGATATTGGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STRADA (92335)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76053
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
63710802 |
+ |
STRADA |
NNNATGGCACGATATTGGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
122934201 |
+ |
AFG2A |
NNNATGGCATGATATTGGGA |
NGG |
1 |
0.9412 |
Tier I |
| 3 |
NC_000005.10 |
5 |
151883310 |
+ |
GLRA1 |
NNNATGGCATGATATTGGGT |
NGG |
2 |
0.5647 |
Tier II |
| 4 |
NC_000008.11 |
8 |
60796280 |
+ |
CHD7 |
NNNATGGAAAGATATTGGGA |
NGG |
2 |
0.5633 |
Tier II |
| 5 |
NC_000022.11 |
22 |
33397090 |
- |
LARGE1 |
NNNATGGCAAGATATTGGGT |
NGG |
2 |
0.52 |
Tier II |
| 6 |
NC_000016.10 |
16 |
11132141 |
- |
CLEC16A |
NNNATGGCACAATCTTGGGA |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000004.12 |
4 |
112085253 |
- |
LINC02945 |
NNNATGGCATGATATAGGGA |
NGG |
2 |
0.8556 |
Tier III |
| 8 |
NC_000006.12 |
6 |
32967494 |
+ |
LOC124901302 |
NNNAGGGCACGATATTGAGA |
NGG |
2 |
0.4431 |
Tier III |
| 9 |
NC_000016.10 |
16 |
11132141 |
- |
LOC105371081 |
NNNATGGCACAATCTTGGGA |
NGG |
2 |
0.2143 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
11861728 |
+ |
Gm34237 |
NNNGTGGCAGGATATTGGGA |
NGG |
2 |
0.2431 |
Tier III |
Other clones with same target sequence:
(none)
