Construct: sgRNA BRDN0001145790

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAATCCCATTCCAAAATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL3 (57147)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76794

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 169868972 - SCYL3 NNNATCCCATTCCAAAATGT NGG 0 1.0 Tier I
2 NC_000001.11 1 185982363 + HMCN1 NNNATCCCATTCCAGAACGT NGG 2 0.2786 Tier I
3 NC_000010.11 10 74959994 + KAT6B NNNATCCCATTCCAATATGT NGC 2 0.0 Tier I
4 NC_000005.10 5 76199312 + SV2C NNNATAACATTCCAAAATGT NGG 2 0.6964 Tier II
5 NC_000018.10 18 34571820 + DTNA NNNACCCCATTCCAAAAAGT NGG 2 0.6667 Tier II
6 NC_000003.12 3 129305306 - HMCES NNNATCACTTTCCAAAATGT NGG 2 0.45 Tier II
7 NC_000016.10 16 81381318 + GAN NNNATCCCCTTCCAGAATGT NGG 2 0.3714 Tier II
8 NC_000003.12 3 133404753 - BFSP2 NNNATCCCAGTGCAAAATGT NGG 2 0.2222 Tier II
9 NC_000003.12 3 1298579 - CNTN6 NNNATCCCATGCAAAAATGT NGG 2 0.1538 Tier II
10 NC_000003.12 3 69392821 + FRMD4B NNNATCCCATTCCAAAAAGG NGG 2 0.1176 Tier II
11 NC_000012.12 12 8515592 - CLEC4D NNNATCCCAGTCCAAAATGG NGG 2 0.0882 Tier II
12 NC_000002.12 2 132661479 + LYPD1 NNNATCCCATTCCATAAGGT NGG 2 0.0667 Tier II
13 NC_000004.12 4 79982401 - ANTXR2 NNNATCTCATTCCAAAATGT NTG 2 0.0317 Tier II
14 NC_000004.12 4 155361073 - MAP9 NNNATCCCATTCCAAAATGT NGT 1 0.0161 Tier II
15 NC_000015.10 15 33933062 + AVEN NNNATCCCATTCAAAAATGT NTG 2 0.015 Tier II
16 NC_000012.12 12 12242152 + LRP6 NNNATCCCATTCCAAAATTT NGT 2 0.0108 Tier II
17 NC_000009.12 9 111004837 + LPAR1 NNNATCCCATTCCAGAATGT NGT 2 0.0105 Tier II
18 NC_000003.12 3 185488059 + MAP3K13 NNNATCCCATTCCAAAATGT NTC 2 0.0 Tier II
19 NC_000009.12 9 13466700 - LOC105375977 NNNATCCCAGTCCAAAATGT NAG 2 0.1296 Tier III
20 NC_000011.10 11 23019248 + LINC02718 NNNATCCCATTCAAAAAGGT NGG 2 0.1282 Tier III
21 NC_000011.10 11 23019248 + LOC124902646 NNNATCCCATTCAAAAAGGT NGG 2 0.1282 Tier III
22 NC_000005.10 5 7909613 - LOC124900936 NNNTTCCCATTCCAAAATGG NGG 2 0.1123 Tier III
23 NC_000011.10 11 41764696 + LINC01499 NNNTTCCCATTCCAAAATGG NGG 2 0.1123 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 56917396 - Lnp1 NNNAGCCCATTCCAAAATGC NGG 2 0.0582 Tier I
2 NC_000080.6 14 21609778 + Kat6b NNNATCCCATTCCAATATGT NGC 2 0.0 Tier I
3 NC_000073.6 7 63625754 + Otud7a NNNATTCCATTCCAAAATGT NGG 1 0.9286 Tier II
4 NC_000069.6 3 142073051 + Bmpr1b NNNATACTATTCCAAAATGT NGG 2 0.8125 Tier II
5 NC_000086.7 X 151425596 - Fam120c NNNTTACCATTCCAAAATGT NGG 2 0.5909 Tier II
6 NC_000086.7 X 105891454 + Atrx NNNATCCCATTATAAAATGT NGG 2 0.5 Tier II
7 NC_000074.6 8 114876662 + Wwox NNNAACCCATTCCAAAAAGT NGG 2 0.3333 Tier II
8 NC_000083.6 17 57921765 + Cntnap5c NNNATGCCATTCCTAAATGT NGG 2 0.2667 Tier II
9 NC_000073.6 7 57632906 - Gabrb3 NNNTTCCCATTCCAAAATGT NAG 2 0.165 Tier II
10 NC_000079.6 13 52614690 - Syk NNNATCCCATCCCAAAATGG NGG 2 0.1176 Tier II
11 NC_000073.6 7 135555438 + Ptpre NNNAGCCCATTCCAAAGTGT NGG 2 0.1129 Tier II
12 NC_000076.6 10 28849445 - Themis NNNTTCCCATTCCAAAATGG NGG 2 0.1123 Tier II
13 NC_000078.6 12 79369143 - Rad51b NNNTTCCCATTCCAAAATGG NGG 2 0.1123 Tier II
14 NC_000082.6 16 22033365 - Senp2 NNNATCCCATTCTAAAATGT NGA 2 0.0486 Tier II
15 NC_000072.6 6 21472735 + Kcnd2 NNNATCCCATTCCAAAACGT NGA 2 0.0298 Tier II
16 NC_000072.6 6 116111033 - Tmcc1 NNNATCCCATTCCTAAATGT NTG 2 0.0208 Tier II
17 NC_000077.6 11 105752010 + Tanc2 NNNATCCCATTCCAAAATGG NGT 2 0.0028 Tier II
18 NC_000068.7 2 53058273 + Fmnl2 NNNATCCCATTCCAACATGT NGT 2 0.0 Tier II
19 NC_000072.6 6 28325886 - Zfp800 NNNATCCCATTCCACCATGT NGG 2 0.0 Tier II
20 NC_000068.7 2 52649907 - Cacnb4 NNNATCCCTTTCCAACATGT NGG 2 0.0 Tier II
21 NC_000081.6 15 11849785 - Npr3 NNNATCTCATTCCAACATGT NGG 2 0.0 Tier II
22 NC_000067.6 1 194890394 - Gm39748 NNNTTCCCATTCCAAAATGT NGG 1 0.6364 Tier III
23 NC_000077.6 11 17958239 + Etaa1os NNNTTACCATTCCAAAATGT NGG 2 0.5909 Tier III
24 NC_000078.6 12 68656592 - Gm31307 NNNATGCCATTCAAAAATGT NGG 2 0.1923 Tier III
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Other clones with same target sequence:

(none)