Construct: sgRNA BRDN0001145791
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGGCATGTTAGTCACGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MST1R (4486)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78051
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
49897593 |
+ |
MST1R |
NNNGCATGTTAGTCACGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
22512113 |
- |
NPIPB5 |
NNNGCATGTTAGTCACGGTG |
NAG |
1 |
0.2593 |
Tier II |
| 3 |
NC_000016.10 |
16 |
30247262 |
+ |
NPIPB13 |
NNNGCATGTTAGTCACGGTG |
NAG |
1 |
0.2593 |
Tier II |
| 4 |
NC_000014.9 |
14 |
51861542 |
+ |
GNG2 |
NNNGCATGTTAGCCTCGGTG |
NGG |
2 |
0.1579 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
52656394 |
+ |
Sepsecs |
NNNACATGTTAGTCACGGTG |
NGG |
1 |
0.9 |
Tier II |
| 2 |
NC_000080.6 |
14 |
64364005 |
+ |
Msra |
NNNGCATGCTAGTCACAGTG |
NGG |
2 |
0.8615 |
Tier II |
| 3 |
NC_000079.6 |
13 |
112814067 |
+ |
Plpp1 |
NNNGCATGTTTGTCACAGTG |
NGG |
2 |
0.2872 |
Tier II |
| 4 |
NC_000067.6 |
1 |
91208260 |
- |
Ramp1 |
NNNACATGTTAGGCACGGTG |
NGG |
2 |
0.2348 |
Tier II |
| 5 |
NC_000075.6 |
9 |
105725578 |
+ |
Col6a6 |
NNNGCACGTTAGGCACGGTG |
NGG |
2 |
0.1793 |
Tier II |
| 6 |
NC_000069.6 |
3 |
28000438 |
- |
Pld1 |
NNNGCATGTTAGTCACTTTG |
NGG |
2 |
0.1667 |
Tier II |
Other clones with same target sequence:
(none)
