Construct: sgRNA BRDN0001145793

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAGCTGAGAGATCACTGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK11A (728642)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75889

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 1721654 - CDK11A NNNGCTGAGAGATCACTGCA NGG 0 1.0 Tier I
2 NC_000001.11 1 1655427 - CDK11B NNNGCTGAGAGATCACCGCA NGG 1 0.7059 Tier I
3 NC_000022.11 22 30264422 - OSM NNNACTGAGAGAGCACTGCA NGG 2 0.2348 Tier I
4 NC_000011.10 11 11331264 + GALNT18 NNNGGTGAGAGGTCACTGCA NGG 2 0.4333 Tier II
5 NC_000022.11 22 19455842 + UFD1 NNNGCTTGGAGATCACTGCA NGG 2 0.419 Tier II
6 NC_000007.14 7 104433469 - LHFPL3 NNNCCTGAGAGACCACTGCA NGG 2 0.418 Tier II
7 NC_000003.12 3 136981870 + IL20RB NNNGCTGGGAGATCACAGCA NGG 2 0.3911 Tier II
8 NC_000001.11 1 61331630 - NFIA NNNGTTGAGAGATCACTCCA NGG 2 0.303 Tier II
9 NC_000011.10 11 69815543 + FGF3 NNNGCAGAGAGCTCACTGCA NGG 2 0.2281 Tier II
10 NC_000001.11 1 154731702 + KCNN3 NNNTCTGAGGGATCACTGCA NGG 2 0.202 Tier II
11 NC_000007.14 7 157658465 + PTPRN2 NNNTCTGAGGGATCACTGCA NGG 2 0.202 Tier II
12 NC_000023.11 X 32211172 + DMD NNNTCTGAGAGATCACAGCA NGG 2 0.1939 Tier II
13 NC_000018.10 18 48786161 + CTIF NNNGCTCAGAGAGCACTGCA NGG 2 0.1793 Tier II
14 NC_000003.12 3 79630936 + ROBO1 NNNGATGAGAGATCTCTGCA NGG 2 0.1143 Tier II
15 NC_000017.11 17 77304607 - SEPTIN9 NNNGCTGAGACATCTCTGCA NGG 2 0.0857 Tier II
16 NC_000012.12 12 22269378 - ST8SIA1 NNNGCTGAGAAATCACTGCA NGT 2 0.0161 Tier II
17 NC_000002.12 2 95278489 + PROM2 NNNGCTGAGAGTTCACTGCA NTG 2 0.013 Tier II
18 NC_000019.10 19 11546237 + CNN1 NNNGCTGAGAGCTGACTGCA NGG 2 0.0 Tier II
19 NC_000002.12 2 172985323 + RAPGEF4 NNNGGTGAGAGATGACTGCA NGG 2 0.0 Tier II
20 NC_000022.11 22 49568893 + MIR3667HG NNNGCAGAGAGGTCACTGCA NGG 2 0.6259 Tier III
21 NC_000002.12 2 133930035 - LOC105373627 NNNGCAGAAAGATCACTGCA NGG 2 0.5571 Tier III
22 NC_000003.12 3 136981870 + IL20RB-AS1 NNNGCTGGGAGATCACAGCA NGG 2 0.3911 Tier III
23 NC_000018.10 18 55764869 - LOC105372130 NNNGGTGAAAGATCACTGCA NGG 2 0.3857 Tier III
24 NC_000001.11 1 219472325 - LYPLAL1-AS1 NNNGGTGAGAGATCACTCCA NGG 2 0.2857 Tier III
25 NC_000011.10 11 19285003 - CSRP3-AS1 NNNCCTGAGACATCACTGCA NGG 2 0.2269 Tier III
26 NC_000008.11 8 127890206 - PVT1 NNNGCTGAGAGGTCCCTGCA NGG 2 0.197 Tier III
27 NC_000018.10 18 67681969 + DSEL-AS1 NNNGTTGAGAGATCTCTGCA NGG 2 0.1273 Tier III
28 NC_000018.10 18 67681969 + LOC105372173 NNNGTTGAGAGATCTCTGCA NGG 2 0.1273 Tier III
29 NC_000003.12 3 64925990 - ADAMTS9-AS2 NNNGATGAGAGATCTCTGCA NGG 2 0.1143 Tier III
30 NC_000003.12 3 64925990 - LOC105377124 NNNGATGAGAGATCTCTGCA NGG 2 0.1143 Tier III
31 NC_000010.11 10 78982092 - ZMIZ1-AS1 NNNGCTGAGAGCTCCCTGCA NGG 2 0.0718 Tier III
32 NC_000012.12 12 127293933 - LOC124903050 NNNCCTGAGAGATCACTGGA NGG 2 0.0662 Tier III
33 NC_000001.11 1 219540077 - LYPLAL1-AS1 NNNGCTGAGAGATCACTGAA NTG 2 0.0167 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 155626832 + Cdk11b NNNGCTGAGAGATCACCGAA NGG 2 0.3025 Tier I
2 NC_000068.7 2 5151161 + Ccdc3 NNNACTGAGAGAACACTGCA NGG 2 0.6231 Tier II
3 NC_000067.6 1 58211671 + Aox4 NNNGCTGAGAGATCACAGCA NGG 1 0.5333 Tier II
4 NC_000084.6 18 62501306 - Htr4 NNNCCTGAGAGATCACTGCA NGG 1 0.5294 Tier II
5 NC_000075.6 9 69083873 - Rora NNNGCTGTGAGATCACTGCA NGG 1 0.4286 Tier II
6 NC_000077.6 11 118331244 + Timp2 NNNGCTGAGAGACCACAGCA NGG 2 0.4211 Tier II
7 NC_000083.6 17 49476810 + Daam2 NNNTCTGAGAAATCACTGCA NGG 2 0.3636 Tier II
8 NC_000083.6 17 8951314 + Pde10a NNNTCTGAGTGATCACTGCA NGG 2 0.3209 Tier II
9 NC_000080.6 14 54989001 - Myh7 NNNGCCGAGAGATCACTGCA NAG 2 0.2357 Tier II
10 NC_000079.6 13 65096012 + Mfsd14b NNNGCTGAGAGATTACTGCA NAG 2 0.1901 Tier II
11 NC_000082.6 16 7211630 - Rbfox1 NNNCCTGAGAGATCACTGCA NAG 2 0.1373 Tier II
12 NC_000070.6 4 139616117 - Iffo2 NNNGCTGTGAGATCCCTGCA NGG 2 0.1169 Tier II
13 NC_000086.7 X 139588331 - Rnf128 NNNGCTGAGAGATCACTGAA NAG 2 0.1111 Tier II
14 NC_000078.6 12 53370233 + Npas3 NNNGCTGAGAGATAATTGCA NGG 2 0.1077 Tier II
15 NC_000080.6 14 75980552 + Gtf2f2 NNNGCTGAGAGATCTCTGAA NGG 2 0.0857 Tier II
16 NC_000077.6 11 106324275 + Scn4a NNNCCTGAGAGATCACGGCA NGG 2 0.0623 Tier II
17 NC_000072.6 6 9100518 + Nxph1 NNNACTGAGAGATCACTGCA NTG 2 0.0351 Tier II
18 NC_000076.6 10 12773769 - Utrn NNNGCTGACAGATCACTGCA NTG 2 0.021 Tier II
19 NC_000079.6 13 36867317 + F13a1 NNNGCTGAGAGATGACTTCA NGG 2 0.0 Tier II
20 NC_000068.7 2 32706663 + Cdk9 NNNGCTGAGAGATGGCTGCA NGG 2 0.0 Tier II
21 NC_000068.7 2 118013007 + Gm13986 NNNGCTGGGGGATCACTGCA NGG 2 0.4074 Tier III
22 NC_000071.6 5 23401752 + 5031425E22Rik NNNGGTGATAGATCACTGCA NGG 2 0.32 Tier III
23 NC_000080.6 14 54989001 - Gm31251 NNNGCCGAGAGATCACTGCA NAG 2 0.2357 Tier III
24 NC_000074.6 8 46733758 - Gm16675 NNNGCAGAGAGATCACTGCA NAG 2 0.2247 Tier III
25 NC_000073.6 7 84215986 - LOC117997403 NNNCCTGAGAGATCACTGCA NGA 2 0.0368 Tier III
26 NC_000079.6 13 36867317 + Gm30489 NNNGCTGAGAGATGACTTCA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)