Construct: sgRNA BRDN0001145794
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATCCTAGTCAACTCCCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K1 (5604)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76210
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
66481788 |
+ |
MAP2K1 |
NNNCCTAGTCAACTCCCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
73294767 |
- |
KHDC1 |
NNNTCCAGTCAACTCCCGTG |
NGG |
2 |
0.7273 |
Tier II |
| 3 |
NC_000007.14 |
7 |
158256605 |
+ |
PTPRN2 |
NNNCCTAGGCAGCTCCCGTG |
NGG |
2 |
0.4471 |
Tier II |
| 4 |
NC_000006.12 |
6 |
25368176 |
- |
CARMIL1 |
NNNCCTAGTCAACTCCAGTA |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000005.10 |
5 |
177489917 |
- |
PDLIM7 |
NNNCCCAGTCATCTCCCGTG |
NGG |
2 |
0.303 |
Tier II |
| 6 |
NC_000006.12 |
6 |
116215746 |
- |
COL10A1 |
NNNCCTAGTCAACTCCCCTG |
NTG |
2 |
0.0186 |
Tier II |
| 7 |
NC_000006.12 |
6 |
116215746 |
- |
NT5DC1 |
NNNCCTAGTCAACTCCCCTG |
NTG |
2 |
0.0186 |
Tier II |
| 8 |
NC_000014.9 |
14 |
81282280 |
- |
STON2 |
NNNCCTAGTCAACTTCCGTG |
NGA |
2 |
0.0046 |
Tier II |
| 9 |
NC_000006.12 |
6 |
73294767 |
- |
C6orf147 |
NNNTCCAGTCAACTCCCGTG |
NGG |
2 |
0.7273 |
Tier III |
| 10 |
NC_000006.12 |
6 |
73294767 |
- |
KHDC1-AS1 |
NNNTCCAGTCAACTCCCGTG |
NGG |
2 |
0.7273 |
Tier III |
| 11 |
NC_000006.12 |
6 |
73294767 |
- |
LOC122539213 |
NNNTCCAGTCAACTCCCGTG |
NGG |
2 |
0.7273 |
Tier III |
| 12 |
NC_000006.12 |
6 |
25368176 |
- |
LOC124901281 |
NNNCCTAGTCAACTCCAGTA |
NGG |
2 |
0.4375 |
Tier III |
| 13 |
NC_000008.11 |
8 |
30028319 |
+ |
MAP2K1P1 |
NNNCATAGTCAGCTCCCGTG |
NGG |
2 |
0.4127 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
141495880 |
- |
Col4a5 |
NNNCCCAGGCAACTCCCGTG |
NGG |
2 |
0.5628 |
Tier II |
| 2 |
NC_000069.6 |
3 |
123648295 |
- |
Ndst3 |
NNNCCTAGTCAACTCCCTTT |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000083.6 |
17 |
56711358 |
- |
Ranbp3 |
NNNCCTAGTGAACCCCCGTG |
NGG |
2 |
0.1111 |
Tier II |
Other clones with same target sequence:
(none)
