Construct: sgRNA BRDN0001145800
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGAGTACATCAAGACGGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IRAK1 (3654)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76418
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
154016479 |
- |
IRAK1 |
NNNAGTACATCAAGACGGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
15256556 |
- |
JARID2 |
NNNAGTACATCAAGAAGGGC |
NGG |
2 |
0.2273 |
Tier II |
| 3 |
NC_000001.11 |
1 |
174640222 |
- |
RABGAP1L |
NNNAGTAAATCAAGATGGGA |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000002.12 |
2 |
106838497 |
- |
ST6GAL2 |
NNNAGTACATCAAGAGGTGA |
NGG |
2 |
0.1026 |
Tier II |
| 5 |
NC_000012.12 |
12 |
112328635 |
+ |
HECTD4 |
NNNAGTACATCATGATGGGA |
NGG |
2 |
0.0923 |
Tier II |
| 6 |
NC_000004.12 |
4 |
126221721 |
- |
LOC105377409 |
NNNAGTACATGAAGAGGGGA |
NGG |
2 |
0.0385 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
46972911 |
- |
Nt5c2 |
NNNAGGACATCAAGACAGGA |
NGG |
2 |
0.5333 |
Tier II |
| 2 |
NC_000079.6 |
13 |
44764664 |
- |
Jarid2 |
NNNAGTACACCAAGACGGGG |
NGG |
2 |
0.4078 |
Tier II |
| 3 |
NC_000072.6 |
6 |
99392103 |
- |
Foxp1 |
NNNAGTATATCAAGACGGGA |
NAG |
2 |
0.2269 |
Tier II |
| 4 |
NC_000073.6 |
7 |
50138687 |
+ |
Nell1 |
NNNAGTACATCAAGAAGGGA |
NGT |
2 |
0.0161 |
Tier II |
Other clones with same target sequence:
(none)
