Construct: sgRNA BRDN0001145803

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCCACGCTCAGGTACTCGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK6 (2870)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75702

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 177433158 - GRK6 NNNCACGCTCAGGTACTCGT NGG 0 1.0 Tier I
2 NC_000006.12 6 33669009 - ITPR3 NNNGATGCTCAGGTACTCGT NGG 2 0.4643 Tier I
3 NC_000008.11 8 144621933 + ARHGAP39 NNNCACGCTCAGGTACTCCT NAG 2 0.1162 Tier II
4 NC_000013.11 13 21319608 - GRK6P1 NNNCATGCTCAGGTACCCGT NGG 2 0.6555 Tier III
5 NC_000013.11 13 21319608 - MIPEPP3 NNNCATGCTCAGGTACCCGT NGG 2 0.6555 Tier III
6 NC_000013.11 13 21319608 - LINC00539 NNNCATGCTCAGGTACCCGT NGG 2 0.6555 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 55451340 - Grk6 NNNCGTGCTCAGGTACTCGT NGG 2 0.6686 Tier I
2 NC_000083.6 17 71874917 + Alk NNNCACGCTCAGGTTCTAGT NGG 2 0.1077 Tier II
3 NC_000068.7 2 32578864 + Pip5kl1 NNNCAGGCTCAGGTACTCGG NGG 2 0.0882 Tier II
Download CSV

Other clones with same target sequence:

(none)