Construct: sgRNA BRDN0001145805
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTAGGTACAGACTGACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TWF1 (5756)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77289
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
43797823 |
- |
TWF1 |
NNNTAGGTACAGACTGACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
43797823 |
- |
TMEM117 |
NNNTAGGTACAGACTGACGT |
NGG |
0 |
1.0 |
Tier II |
| 3 |
NC_000023.11 |
X |
155290885 |
- |
CLIC2 |
NNNGAGGTACAGACTGACGT |
NGG |
1 |
0.625 |
Tier II |
| 4 |
NC_000009.12 |
9 |
132539204 |
+ |
CFAP77 |
NNNTAGGTCCAGACTGACGA |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000004.12 |
4 |
30809666 |
- |
PCDH7 |
NNNTAGGTTCAGAATGACGT |
NGG |
2 |
0.21 |
Tier II |
| 6 |
NC_000001.11 |
1 |
52310472 |
+ |
ZFYVE9 |
NNNTAGGTACAGGCTGAGGT |
NGG |
2 |
0.087 |
Tier II |
| 7 |
NC_000017.11 |
17 |
61156527 |
+ |
BCAS3 |
NNNTAGGTTCAGACTGAGGT |
NGG |
2 |
0.08 |
Tier II |
| 8 |
NC_000009.12 |
9 |
130915041 |
- |
FIBCD1 |
NNNTAGGTCCAGACTGTCGT |
NGG |
2 |
0.0762 |
Tier II |
| 9 |
NC_000021.9 |
21 |
26424986 |
+ |
CYYR1-AS1 |
NNNTAAGAACAGACTGACGT |
NGG |
2 |
0.8 |
Tier III |
| 10 |
NC_000017.11 |
17 |
29203981 |
- |
TWF1P1 |
NNNGAGGTACAGACTGACGT |
NGG |
1 |
0.625 |
Tier III |
| 11 |
NC_000023.11 |
X |
155290885 |
- |
TWF1P2 |
NNNGAGGTACAGACTGACGT |
NGG |
1 |
0.625 |
Tier III |
| 12 |
NC_000023.11 |
X |
98576002 |
+ |
LINC03077 |
NNNTAGGTACTGACTGATGT |
NGG |
2 |
0.1978 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
43040985 |
- |
Tenm1 |
NNNTAGATACAGACTGACAT |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000070.6 |
4 |
154968373 |
- |
Pank4 |
NNNGAGGTGCAGACTGACGT |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000081.6 |
15 |
23088573 |
+ |
Cdh18 |
NNNTAGTTACAGACTGATGT |
NGG |
2 |
0.3673 |
Tier II |
| 4 |
NC_000070.6 |
4 |
111609197 |
+ |
Agbl4 |
NNNAAGGTACAGACTGAAGT |
NGG |
2 |
0.2564 |
Tier II |
| 5 |
NC_000078.6 |
12 |
108675054 |
- |
Evl |
NNNTAGGTACTGACTGAAGT |
NGG |
2 |
0.1657 |
Tier II |
| 6 |
NC_000081.6 |
15 |
52035148 |
+ |
Gm19236 |
NNNTAGGTACAAACTGACGT |
NTG |
2 |
0.0364 |
Tier III |
Other clones with same target sequence:
(none)
