Construct: sgRNA BRDN0001145809

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CACAAAAACGGGGTTACGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK14 (1432)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77923

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 36052728 + MAPK14 NNNAAAAACGGGGTTACGTG NGG 0 1.0 Tier I
2 NC_000011.10 11 1297583 - TOLLIP NNNAAAAACGGGGTAAAGTG NGG 2 0.2702 Tier II
3 NC_000017.11 17 160293 + DOC2B NNNAAAAACCGGGTTACCTG NGG 2 0.1905 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 4714928 + Gria4 NNNAAAAACGGGGATATGTG NGG 2 0.2889 Tier II
2 NC_000080.6 14 12146537 - Ptprg NNNAAAAACGGGGTTAAGAG NGG 2 0.1333 Tier II
3 NC_000079.6 13 40871488 + Gcnt2 NNNAAAAACGGGGAGACGTG NGG 2 0.031 Tier II
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Other clones with same target sequence:

(none)