Construct: sgRNA BRDN0001145815
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTATGTATTGGCGTTGAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FER (2241)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76801
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
108867854 |
+ |
FER |
NNNTGTATTGGCGTTGAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
6728142 |
+ |
COPS7A |
NNNTGAATTGGCATTGAAAG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000003.12 |
3 |
38850010 |
+ |
SCN11A |
NNNTGTATAGGGGTTGAAAG |
NGG |
2 |
0.4127 |
Tier II |
| 4 |
NC_000011.10 |
11 |
36434963 |
+ |
PRR5L |
NNNTGTCTTGGCATTGAAAG |
NGG |
2 |
0.4038 |
Tier II |
| 5 |
NC_000002.12 |
2 |
134648124 |
+ |
TMEM163 |
NNNTTTATTGGCGATGAAAG |
NGG |
2 |
0.1857 |
Tier II |
| 6 |
NC_000023.11 |
X |
138849785 |
+ |
FGF13 |
NNNTGTCTTGGCCTTGAAAG |
NGG |
2 |
0.1842 |
Tier II |
| 7 |
NC_000012.12 |
12 |
9069054 |
- |
A2M |
NNNTGTATTGGCATTGACAG |
NGG |
2 |
0.1758 |
Tier II |
| 8 |
NC_000012.12 |
12 |
9069054 |
- |
KLRG1 |
NNNTGTATTGGCATTGACAG |
NGG |
2 |
0.1758 |
Tier II |
| 9 |
NC_000008.11 |
8 |
29244450 |
- |
KIF13B |
NNNTGTATTGGCCCTGAAAG |
NGG |
2 |
0.1203 |
Tier II |
| 10 |
NC_000002.12 |
2 |
132480487 |
+ |
GPR39 |
NNNTGTATTGGGGTTGAAAG |
NGA |
2 |
0.0309 |
Tier II |
| 11 |
NC_000014.9 |
14 |
34169116 |
- |
LOC102724945 |
NNNTGTCTTGGAGTTGAAAG |
NGG |
2 |
0.3125 |
Tier III |
| 12 |
NC_000005.10 |
5 |
33038731 |
- |
LOC105374715 |
NNNTGTATTGGAGTTGAAAC |
NGG |
2 |
0.3061 |
Tier III |
| 13 |
NC_000003.12 |
3 |
106812943 |
- |
LOC101929534 |
NNNTGTATTGGGGTGGAAAG |
NGG |
2 |
0.0222 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
19982977 |
+ |
Spred2 |
NNNTGCCTTGGCGTTGAAAG |
NGG |
2 |
0.3977 |
Tier II |
| 2 |
NC_000076.6 |
10 |
107353231 |
- |
Lin7a |
NNNTGTGTTGGTGTTGAAAG |
NGG |
2 |
0.3801 |
Tier II |
| 3 |
NC_000071.6 |
5 |
44488979 |
- |
Ldb2 |
NNNTGTATTGGCTTTGAAAA |
NGG |
2 |
0.2813 |
Tier II |
| 4 |
NC_000068.7 |
2 |
154846769 |
+ |
Raly |
NNNTGTATTGGCCATGAAAG |
NGG |
2 |
0.2607 |
Tier II |
| 5 |
NC_000084.6 |
18 |
75587445 |
- |
Ctif |
NNNTGTATGGTCGTTGAAAG |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000077.6 |
11 |
77014606 |
+ |
Slc6a4 |
NNNTGTATTGGCTTTGTAAG |
NGG |
2 |
0.04 |
Tier II |
| 7 |
NC_000068.7 |
2 |
153780694 |
- |
Efcab8 |
NNNTGTATTGGCCTTGAAAG |
NGA |
2 |
0.0292 |
Tier II |
Other clones with same target sequence:
(none)
