Construct: sgRNA BRDN0001145816
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGTCATTCTCAGGGACACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK2 (10110)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76206
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
43572055 |
+ |
SGK2 |
NNNTCATTCTCAGGGACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
90707084 |
+ |
TTC7B |
NNNTCATTCTCAAGGACACA |
NGG |
2 |
0.8654 |
Tier II |
| 3 |
NC_000023.11 |
X |
71245268 |
+ |
ZMYM3 |
NNNTCATGCTCAGGGACACA |
NGG |
2 |
0.6875 |
Tier II |
| 4 |
NC_000006.12 |
6 |
110746774 |
+ |
CDK19 |
NNNTCATTCTCAGGAACACT |
NGG |
2 |
0.6588 |
Tier II |
| 5 |
NC_000002.12 |
2 |
230930204 |
+ |
GPR55 |
NNNTATTTCTCAGGGACACG |
NGG |
2 |
0.4082 |
Tier II |
| 6 |
NC_000001.11 |
1 |
176674063 |
+ |
PAPPA2 |
NNNACATTCTCAGGGACACT |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000009.12 |
9 |
84367936 |
+ |
SLC28A3 |
NNNCCATTCTCAGGGACATG |
NGG |
2 |
0.2986 |
Tier II |
| 8 |
NC_000009.12 |
9 |
134656395 |
+ |
COL5A1 |
NNNTGATTCTCAGGGACACC |
NGG |
2 |
0.2571 |
Tier II |
| 9 |
NC_000006.12 |
6 |
138583057 |
- |
NHSL1 |
NNNTCATTTTCAGGCACACG |
NGG |
2 |
0.2386 |
Tier II |
| 10 |
NC_000007.14 |
7 |
154056583 |
+ |
DPP6 |
NNNTCATTTTCAGTGACACG |
NGG |
2 |
0.2333 |
Tier II |
| 11 |
NC_000010.11 |
10 |
12575728 |
+ |
CAMK1D |
NNNTCATTCCCAGGGACCCG |
NGG |
2 |
0.1016 |
Tier II |
| 12 |
NC_000011.10 |
11 |
45655900 |
+ |
CHST1 |
NNNTCATTCTCAGGGACAGT |
NGG |
2 |
0.0875 |
Tier II |
| 13 |
NC_000006.12 |
6 |
154481609 |
- |
CNKSR3 |
NNNTCATTCTCAGGGAGACA |
NGG |
2 |
0.0551 |
Tier II |
| 14 |
NC_000015.10 |
15 |
77772205 |
- |
LINGO1 |
NNNTCATTCTCAGGGACAGG |
NAG |
2 |
0.0324 |
Tier II |
| 15 |
NC_000010.11 |
10 |
126320265 |
+ |
ADAM12 |
NNNACATTCTCAGGGACACG |
NGC |
2 |
0.0106 |
Tier II |
| 16 |
NC_000007.14 |
7 |
1556192 |
+ |
TMEM184A |
NNNTCATTCTCATGGCCACG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000009.12 |
9 |
84452951 |
+ |
LOC102724036 |
NNNTCATTCACAGGGAAACG |
NGG |
2 |
0.4 |
Tier III |
| 18 |
NC_000009.12 |
9 |
84452951 |
+ |
LOC124902192 |
NNNTCATTCACAGGGAAACG |
NGG |
2 |
0.4 |
Tier III |
| 19 |
NC_000007.14 |
7 |
143467701 |
- |
EPHA1-AS1 |
NNNTCATACTCAGGGACAAG |
NGG |
2 |
0.3429 |
Tier III |
| 20 |
NC_000007.14 |
7 |
154056583 |
+ |
LOC101929998 |
NNNTCATTTTCAGTGACACG |
NGG |
2 |
0.2333 |
Tier III |
| 21 |
NC_000011.10 |
11 |
1335335 |
- |
LOC124902606 |
NNNTCATTCTCAGGGACAGG |
NGG |
1 |
0.125 |
Tier III |
| 22 |
NC_000002.12 |
2 |
233603709 |
+ |
UGT1A |
NNNTCATTCTCAGGGGCATG |
NGG |
2 |
0.0888 |
Tier III |
| 23 |
NC_000002.12 |
2 |
233603709 |
+ |
UGT1A11P |
NNNTCATTCTCAGGGGCATG |
NGG |
2 |
0.0888 |
Tier III |
| 24 |
NC_000021.9 |
21 |
43430118 |
+ |
LOC124905029 |
NNNTCCTTCTCAGGGACCCG |
NGG |
2 |
0.0866 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
97506637 |
- |
Dcn |
NNNTCATTCTCATGGACACG |
NAG |
2 |
0.0778 |
Tier I |
| 2 |
NC_000068.7 |
2 |
155012371 |
+ |
a |
NNNTCATTCTCGGGGACACG |
NGG |
1 |
0.7222 |
Tier II |
| 3 |
NC_000072.6 |
6 |
119083692 |
- |
Cacna1c |
NNNTCTTTCTCAGGGACACG |
NGG |
1 |
0.7143 |
Tier II |
| 4 |
NC_000069.6 |
3 |
53591614 |
+ |
Frem2 |
NNNTCAGTCTCAGGAACACG |
NGG |
2 |
0.5536 |
Tier II |
| 5 |
NC_000075.6 |
9 |
25535769 |
+ |
Eepd1 |
NNNTCATTCTCGGGGACACT |
NGG |
2 |
0.5056 |
Tier II |
| 6 |
NC_000085.6 |
19 |
4466971 |
+ |
Syt12 |
NNNTCATCCTCAGGGACTCG |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000078.6 |
12 |
51719839 |
+ |
Ap4s1 |
NNNTCATTCTCAGGGACATT |
NGG |
2 |
0.3231 |
Tier II |
| 8 |
NC_000072.6 |
6 |
5391237 |
- |
Asb4 |
NNNTCATTCACAGGGACACG |
NAG |
2 |
0.2222 |
Tier II |
| 9 |
NC_000078.6 |
12 |
105778302 |
- |
Ak7 |
NNNTCATCCTCAGGGGCACG |
NGG |
2 |
0.1923 |
Tier II |
| 10 |
NC_000071.6 |
5 |
30667348 |
+ |
Cenpa |
NNNTCATTCTGACGGACACG |
NGG |
2 |
0.1053 |
Tier II |
| 11 |
NC_000068.7 |
2 |
29687710 |
+ |
Rapgef1 |
NNNTTATTCTCAGGGACAGG |
NGG |
2 |
0.0795 |
Tier II |
| 12 |
NC_000072.6 |
6 |
101370180 |
- |
Pdzrn3 |
NNNTCATTCTCAGGGAGACC |
NGG |
2 |
0.0252 |
Tier II |
| 13 |
NC_000077.6 |
11 |
79639298 |
- |
Rab11fip4 |
NNNTCAGTCTCAGGGACACG |
NTG |
2 |
0.0229 |
Tier II |
| 14 |
NC_000068.7 |
2 |
144258459 |
+ |
Snx5 |
NNNTCATTCTCAGGGACATG |
NGC |
2 |
0.0103 |
Tier II |
| 15 |
NC_000073.6 |
7 |
78163413 |
- |
Gm46012 |
NNNTCATTATCAGGGACACA |
NGG |
2 |
0.8036 |
Tier III |
| 16 |
NC_000067.6 |
1 |
75371836 |
- |
Gm15179 |
NNNTCATTCTCAGCGACATG |
NGG |
2 |
0.1978 |
Tier III |
| 17 |
NC_000079.6 |
13 |
83345606 |
+ |
Gm33217 |
NNNTCATTCTGAGGGACAAG |
NGG |
2 |
0.1071 |
Tier III |
| 18 |
NC_000084.6 |
18 |
6004574 |
+ |
Gm41663 |
NNNTCATTCTCAGGGACAAG |
NGA |
2 |
0.0298 |
Tier III |
| 19 |
NC_000077.6 |
11 |
79639298 |
- |
Gm30718 |
NNNTCAGTCTCAGGGACACG |
NTG |
2 |
0.0229 |
Tier III |
| 20 |
NC_000078.6 |
12 |
85453581 |
- |
Gm38543 |
NNNTCATTCTCAGGGACGCG |
NTG |
2 |
0.0156 |
Tier III |
Other clones with same target sequence:
(none)
