Construct: sgRNA BRDN0001145818
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCGAATTCGACGAGGTCAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAR2A (5576)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77672
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48847516 |
+ |
PRKAR2A |
NNNAATTCGACGAGGTCAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
32498606 |
- |
MYO1D |
NNNAATTCCACGAGGGCAGG |
NGG |
2 |
0.1864 |
Tier II |
| 3 |
NC_000017.11 |
17 |
32498606 |
- |
LOC124903982 |
NNNAATTCCACGAGGGCAGG |
NGG |
2 |
0.1864 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
108692538 |
- |
Prkar2a |
NNNAAGTCGACGAGGTCGGG |
NGG |
2 |
0.2286 |
Tier I |
| 2 |
NC_000080.6 |
14 |
28146338 |
- |
Erc2 |
NNNAATTTGACAAGGTCAGG |
NGG |
2 |
0.8167 |
Tier II |
| 3 |
NC_000082.6 |
16 |
25999084 |
- |
P3h2 |
NNNACTTCGACGTGGTCAGG |
NGG |
2 |
0.15 |
Tier II |
| 4 |
NC_000082.6 |
16 |
75150657 |
- |
Robo2 |
NNNAATTCCAGGAGGTCAGG |
NGG |
2 |
0.1346 |
Tier II |
| 5 |
NC_000071.6 |
5 |
134996966 |
+ |
Wbscr25 |
NNNAATTCAACGAGGTCAAG |
NGG |
2 |
0.4592 |
Tier III |
| 6 |
NC_000079.6 |
13 |
88533690 |
- |
Gm34585 |
NNNAATTCGAGGAGGTGAGG |
NGG |
2 |
0.0147 |
Tier III |
Other clones with same target sequence:
(none)
