Construct: sgRNA BRDN0001145840

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGCGGCGTAATGCTTGAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80185

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 71289800 + GALNT17 NNNGGAGTATTGCTTGAAAG NGG 2 0.8193 Tier II
2 NC_000021.9 21 32764891 - PAXBP1 NNNGAAGTAATGCTTGAAAG NGG 2 0.8048 Tier II
3 NC_000001.11 1 165553702 + LRRC52 NNNGGGGTAATCCTTGAAAG NGG 2 0.2647 Tier II
4 NC_000012.12 12 4531671 - C12orf4 NNNGGCGTTATGCCTGAAAG NGG 2 0.1714 Tier II
5 NC_000001.11 1 165553702 + LRRC52-AS1 NNNGGGGTAATCCTTGAAAG NGG 2 0.2647 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 129445895 - Thsd7b NNNGGCAAAATGCTTGAAAG NGG 2 0.8 Tier II
Download CSV

Other clones with same target sequence:

(none)