Construct: sgRNA BRDN0001145847
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTAGCAGTCCCATTAAGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK12 (51755)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75917
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
39501287 |
- |
CDK12 |
NNNGCAGTCCCATTAAGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
35079834 |
+ |
ISX |
NNNGCAGTCCAATTAAGACA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000003.12 |
3 |
43407379 |
- |
ANO10 |
NNNGCAGTCTCTTTAAGTCA |
NGG |
2 |
0.3137 |
Tier II |
| 4 |
NC_000016.10 |
16 |
21748570 |
+ |
OTOA |
NNNACAGTCCCATTAAGTCA |
NAG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000001.11 |
1 |
245916357 |
+ |
SMYD3 |
NNNTGAGTCCCATTAAGTCA |
NGG |
2 |
0.2182 |
Tier II |
| 6 |
NC_000007.14 |
7 |
50052989 |
- |
ZPBP |
NNNGCAGTCCCATCAAGACA |
NGG |
2 |
0.1905 |
Tier II |
| 7 |
NC_000005.10 |
5 |
79785770 |
- |
CMYA5 |
NNNGCATTCCCATTAAGTCA |
NAG |
2 |
0.1481 |
Tier II |
| 8 |
NC_000009.12 |
9 |
116702617 |
- |
ASTN2 |
NNNGGAGTCCCATTAAGTCA |
NGT |
2 |
0.0097 |
Tier II |
| 9 |
NC_000007.14 |
7 |
86768340 |
+ |
GRM3 |
NNNGCAGTCCCATGAAGTCA |
NTG |
2 |
0.0 |
Tier II |
| 10 |
NC_000003.12 |
3 |
71096628 |
+ |
FOXP1 |
NNNGCAGTCCCATTATGTCT |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000021.9 |
21 |
19917815 |
+ |
LOC105372745 |
NNNGCAGTTCCATTAAGTCT |
NGG |
2 |
0.525 |
Tier III |
| 12 |
NC_000016.10 |
16 |
22564693 |
+ |
OTOAP1 |
NNNACAGTCCCATTAAGTCA |
NAG |
2 |
0.2333 |
Tier III |
| 13 |
NC_000003.12 |
3 |
127330186 |
+ |
LINC02016 |
NNNGCAGTCCCATGAGGTCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
98224479 |
- |
Cdk12 |
NNNGCAGTCCCATTAAGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
129037480 |
+ |
Vinac1 |
NNNGGTGTCCCATTAAGTCA |
NGG |
2 |
0.4286 |
Tier I |
| 3 |
NC_000079.6 |
13 |
14419791 |
- |
Hecw1 |
NNNGCAATCCCATTAAATCA |
NGG |
2 |
0.9333 |
Tier II |
| 4 |
NC_000076.6 |
10 |
87546431 |
- |
Pah |
NNNGCGGTCCCATTAAGTCA |
NGG |
1 |
0.7143 |
Tier II |
| 5 |
NC_000084.6 |
18 |
53537891 |
+ |
Prdm6 |
NNNGCAGTGTCATTAAGTCA |
NGG |
2 |
0.5826 |
Tier II |
| 6 |
NC_000070.6 |
4 |
36084241 |
+ |
Lingo2 |
NNNGCAGTCCAGTTAAGTCA |
NGG |
2 |
0.5417 |
Tier II |
| 7 |
NC_000076.6 |
10 |
73659457 |
- |
Pcdh15 |
NNNGAAGTCCCATTAAGTAA |
NGG |
2 |
0.2449 |
Tier II |
| 8 |
NC_000071.6 |
5 |
114728223 |
- |
Git2 |
NNNGCAGTCCCGTTAAGTCA |
NGA |
2 |
0.0502 |
Tier II |
| 9 |
NC_000070.6 |
4 |
102818607 |
- |
Sgip1 |
NNNGCATTCCCATGAAGTCA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000073.6 |
7 |
17439446 |
+ |
Gm32846 |
NNNGCAATCCCATTAAATCA |
NGG |
2 |
0.9333 |
Tier III |
| 11 |
NC_000077.6 |
11 |
11660436 |
- |
Gm11999 |
NNNGCAGTCCCATTAAGTTA |
NAG |
2 |
0.1197 |
Tier III |
| 12 |
NC_000067.6 |
1 |
17107186 |
+ |
Gm31982 |
NNNGCAGTCCCAGTCAGTCA |
NGG |
2 |
0.0711 |
Tier III |
Other clones with same target sequence:
(none)
