Construct: sgRNA BRDN0001145848
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAATATACCGAAGTTCAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK3 (9451)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77166
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
88591029 |
+ |
EIF2AK3 |
NNNTATACCGAAGTTCAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
85705039 |
+ |
SYTL2 |
NNNTATACTGAAGTTCAAAG |
NAG |
2 |
0.2269 |
Tier I |
| 3 |
NC_000018.10 |
18 |
45196224 |
+ |
SLC14A2 |
NNNTACACTGAAGTTCAAAG |
NGG |
2 |
0.7955 |
Tier II |
| 4 |
NC_000006.12 |
6 |
101757593 |
+ |
GRIK2 |
NNNTATACTGAGGTTCAAAG |
NGG |
2 |
0.6319 |
Tier II |
| 5 |
NC_000002.12 |
2 |
30594334 |
+ |
LCLAT1 |
NNNTATAGCAAAGTTCAAAG |
NGG |
2 |
0.6 |
Tier II |
| 6 |
NC_000016.10 |
16 |
12958755 |
+ |
SHISA9 |
NNNCATACTGAAGTTCAAAG |
NGG |
2 |
0.5662 |
Tier II |
| 7 |
NC_000007.14 |
7 |
3936749 |
+ |
SDK1 |
NNNTATACCAAAGTACAAAG |
NGG |
2 |
0.5404 |
Tier II |
| 8 |
NC_000010.11 |
10 |
76982738 |
+ |
KCNMA1 |
NNNAATACTGAAGTTCAAAG |
NGG |
2 |
0.4167 |
Tier II |
| 9 |
NC_000011.10 |
11 |
12469148 |
+ |
PARVA |
NNNTATACCGAAGTTCAAGG |
NGG |
1 |
0.375 |
Tier II |
| 10 |
NC_000003.12 |
3 |
71028286 |
- |
FOXP1 |
NNNTCTACCGAAGTTCAAAG |
NGA |
2 |
0.0347 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
70881508 |
- |
Eif2ak3 |
NNNTATACCGAAGTTCAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
104075232 |
+ |
Gm17660 |
NNNTATAAAGAAGTTCAAAG |
NGG |
2 |
0.5571 |
Tier II |
| 3 |
NC_000086.7 |
X |
60083487 |
+ |
Mcf2 |
NNNGAAACCGAAGTTCAAAG |
NGG |
2 |
0.5417 |
Tier II |
| 4 |
NC_000086.7 |
X |
42825622 |
- |
Tenm1 |
NNNTATCCAGAAGTTCAAAG |
NGG |
2 |
0.375 |
Tier II |
| 5 |
NC_000068.7 |
2 |
77652180 |
- |
Zfp385b |
NNNTATACTGAAGTTCAGAG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000070.6 |
4 |
113066158 |
- |
Skint6 |
NNNTATACAGCAGTTCAAAG |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000069.6 |
3 |
158516651 |
- |
Lrrc7 |
NNNTATACAGAAGTTTAAAG |
NGG |
2 |
0.2637 |
Tier II |
| 8 |
NC_000069.6 |
3 |
152257928 |
- |
Nexn |
NNNTATACAGAAGCTCAAAG |
NGG |
2 |
0.2449 |
Tier II |
Other clones with same target sequence:
(none)
