Construct: sgRNA BRDN0001145850
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACCCCGAGCTTGGGTACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK1 (8408)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75737
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
131910735 |
- |
ULK1 |
NNNCCCGAGCTTGGGTACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
70638325 |
+ |
AUTS2 |
NNNCCCCAGCTTGGGTACCA |
NGG |
2 |
0.3082 |
Tier II |
| 3 |
NC_000021.9 |
21 |
6447630 |
- |
LOC102724560 |
NNNCCCAAGCTTGGGTAGGA |
NGG |
2 |
0.1333 |
Tier II |
| 4 |
NC_000021.9 |
21 |
43055952 |
- |
CBS |
NNNCCCAAGCTTGGGTAGGA |
NGG |
2 |
0.1333 |
Tier II |
| 5 |
NC_000015.10 |
15 |
41888842 |
+ |
SPTBN5 |
NNNCCAGAGCTTGGGTAGGA |
NGG |
2 |
0.1238 |
Tier II |
| 6 |
NC_000001.11 |
1 |
180912684 |
- |
LINC02816 |
NNNCCCGAGCTTGGGGACGA |
NGG |
1 |
0.3462 |
Tier III |
| 7 |
NC_000021.9 |
21 |
6447630 |
- |
LOC102724701 |
NNNCCCAAGCTTGGGTAGGA |
NGG |
2 |
0.1333 |
Tier III |
| 8 |
NC_000008.11 |
8 |
124835357 |
- |
LOC105375743 |
NNNCCCGAGCTTGGGTTTGA |
NGG |
2 |
0.0857 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
145094761 |
- |
Clca2 |
NNNGCAGAGCTTGGGTACGA |
NGG |
2 |
0.4643 |
Tier II |
Other clones with same target sequence:
(none)
