Construct: sgRNA BRDN0001145851
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGGACCTGGATACTCTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IPPK (64768)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77151
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
92649544 |
- |
IPPK |
NNNGACCTGGATACTCTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
36684513 |
+ |
CDKN1A |
NNNGACCTGGAGACTCTCAG |
NGT |
2 |
0.0081 |
Tier I |
| 3 |
NC_000023.11 |
X |
104659217 |
+ |
IL1RAPL2 |
NNNGACCAGTATACTCTCAG |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000015.10 |
15 |
82688968 |
- |
AP3B2 |
NNNGACCCGGATACACTCAG |
NGG |
2 |
0.5789 |
Tier II |
| 5 |
NC_000015.10 |
15 |
25683966 |
+ |
ATP10A |
NNNGACCTGCATACTCTCTG |
NGG |
2 |
0.2154 |
Tier II |
| 6 |
NC_000003.12 |
3 |
37540854 |
- |
ITGA9 |
NNNGACCTGGAAACTCTCAG |
NAG |
2 |
0.2074 |
Tier II |
| 7 |
NC_000009.12 |
9 |
259195 |
+ |
DOCK8 |
NNNGTCCTGGCTACTCTCAG |
NGG |
2 |
0.1455 |
Tier II |
| 8 |
NC_000018.10 |
18 |
79435693 |
- |
NFATC1 |
NNNCACCTGGATCCTCTCAG |
NGG |
2 |
0.1115 |
Tier II |
| 9 |
NC_000009.12 |
9 |
89044887 |
- |
SHC3 |
NNNGCCCTGGATACTCTCAG |
NGA |
2 |
0.0347 |
Tier II |
| 10 |
NC_000005.10 |
5 |
177364132 |
+ |
RGS14 |
NNNGACCTGAATACTCTCAG |
NGT |
2 |
0.0151 |
Tier II |
| 11 |
NC_000013.11 |
13 |
21103728 |
- |
IPPKP1 |
NNNGACCTGGACACTCTCAG |
NGG |
1 |
0.9474 |
Tier III |
| 12 |
NC_000004.12 |
4 |
159551525 |
+ |
LOC107986324 |
NNNGACCAGGATACTATCAG |
NGG |
2 |
0.8 |
Tier III |
| 13 |
NC_000015.10 |
15 |
82688968 |
- |
CPEB1-AS1 |
NNNGACCCGGATACACTCAG |
NGG |
2 |
0.5789 |
Tier III |
| 14 |
NC_000012.12 |
12 |
118915427 |
- |
LOC105370019 |
NNNGACCTGGAAACTCACAG |
NGG |
2 |
0.4267 |
Tier III |
| 15 |
NC_000018.10 |
18 |
79435693 |
- |
LOC124904334 |
NNNCACCTGGATCCTCTCAG |
NGG |
2 |
0.1115 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
65923258 |
+ |
Dnah9 |
NNNAACCTGGATACTCTTAG |
NGG |
2 |
0.5786 |
Tier II |
| 2 |
NC_000084.6 |
18 |
67140078 |
- |
Gnal |
NNNGACCTGGACACACTCAG |
NGG |
2 |
0.5485 |
Tier II |
| 3 |
NC_000076.6 |
10 |
94466837 |
+ |
Tmcc3 |
NNNGACCAGGATGCTCTCAG |
NGG |
2 |
0.5217 |
Tier II |
| 4 |
NC_000085.6 |
19 |
56460299 |
- |
Plekhs1 |
NNNGACCTGGAAACTCTCTG |
NGG |
2 |
0.4308 |
Tier II |
| 5 |
NC_000080.6 |
14 |
57586183 |
- |
Xpo4 |
NNNGACCTGCAAACTCTCAG |
NGG |
2 |
0.32 |
Tier II |
| 6 |
NC_000078.6 |
12 |
76170018 |
- |
Esr2 |
NNNGACCTGGATTCTCTCAA |
NGG |
2 |
0.2813 |
Tier II |
| 7 |
NC_000075.6 |
9 |
119978237 |
+ |
Csrnp1 |
NNNGACCTGGAGACCCTCAG |
NGG |
2 |
0.1364 |
Tier II |
| 8 |
NC_000072.6 |
6 |
12684338 |
+ |
Thsd7a |
NNNGACCTGGATTCTCTCAG |
NGA |
2 |
0.0208 |
Tier II |
| 9 |
NC_000068.7 |
2 |
97452680 |
+ |
Lrrc4c |
NNNGACCTGGATAGTCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000085.6 |
19 |
33241001 |
+ |
Rnls |
NNNGCACTGGATACTCTCAG |
NGG |
2 |
0.4643 |
Tier III |
| 11 |
NC_000080.6 |
14 |
63370143 |
+ |
Gm36165 |
NNNGTCCTGGATACTCTCAA |
NGG |
2 |
0.3409 |
Tier III |
| 12 |
NC_000080.6 |
14 |
63370143 |
+ |
Gm46458 |
NNNGTCCTGGATACTCTCAA |
NGG |
2 |
0.3409 |
Tier III |
| 13 |
NC_000069.6 |
3 |
60815645 |
- |
Gm553061 |
NNNGACCTTGATACTCTCAG |
NGA |
2 |
0.037 |
Tier III |
Other clones with same target sequence:
(none)
