Construct: sgRNA BRDN0001145857
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAACATCTATTGAGACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TXK (7294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76358
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
48104906 |
- |
TXK |
NNNACATCTATTGAGACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
22601462 |
+ |
ABHD4 |
NNNATATCTATTGAAACAAG |
NGG |
2 |
0.5989 |
Tier II |
| 3 |
NC_000003.12 |
3 |
75954013 |
- |
ROBO2 |
NNNACATCTATAGAGACAAT |
NGG |
2 |
0.56 |
Tier II |
| 4 |
NC_000006.12 |
6 |
119076416 |
- |
FAM184A |
NNNATATCTAATGAGACAAG |
NGG |
2 |
0.4773 |
Tier II |
| 5 |
NC_000023.11 |
X |
135537359 |
+ |
INTS6L |
NNNACATTTATTGAGACAGG |
NGG |
2 |
0.3281 |
Tier II |
| 6 |
NC_000012.12 |
12 |
132691861 |
- |
PXMP2 |
NNNACATCTACTGGGACAAG |
NGG |
2 |
0.3111 |
Tier II |
| 7 |
NC_000003.12 |
3 |
16976107 |
+ |
PLCL2 |
NNNACAGCTATTGAGAAAAG |
NGG |
2 |
0.2745 |
Tier II |
| 8 |
NC_000003.12 |
3 |
172369663 |
- |
FNDC3B |
NNNACAGCTATTGAGAAAAG |
NGG |
2 |
0.2745 |
Tier II |
| 9 |
NC_000009.12 |
9 |
5887695 |
+ |
BRD10 |
NNNAAATCTATTGACACAAG |
NGG |
2 |
0.1558 |
Tier II |
| 10 |
NC_000011.10 |
11 |
113042240 |
- |
NCAM1 |
NNNACATCTCTTCAGACAAG |
NGG |
2 |
0.1404 |
Tier II |
| 11 |
NC_000004.12 |
4 |
53305747 |
- |
SCFD2 |
NNNACATCTATTGATACATG |
NGG |
2 |
0.0769 |
Tier II |
| 12 |
NC_000016.10 |
16 |
26027832 |
+ |
HS3ST4 |
NNNACATCTAGTGATACAAG |
NGG |
2 |
0.0571 |
Tier II |
| 13 |
NC_000022.11 |
22 |
17398529 |
+ |
CECR2 |
NNNACATTTATTGAGAGAAG |
NGG |
2 |
0.0515 |
Tier II |
| 14 |
NC_000018.10 |
18 |
49867329 |
+ |
MYO5B |
NNNTCATCTATTGAGACAAG |
NTG |
2 |
0.0248 |
Tier II |
| 15 |
NC_000012.12 |
12 |
103505214 |
+ |
C12orf42 |
NNNACATCTATTGAGAGAAG |
NAG |
2 |
0.0153 |
Tier II |
| 16 |
NC_000004.12 |
4 |
77755219 |
+ |
CNOT6L |
NNNACATCTATAGAGACAAG |
NGT |
2 |
0.0129 |
Tier II |
| 17 |
NC_000013.11 |
13 |
109069862 |
- |
MYO16 |
NNNACATCTATTGAGATAAG |
NGT |
2 |
0.0075 |
Tier II |
| 18 |
NC_000012.12 |
12 |
93361187 |
- |
LOC643339 |
NNNACATTTGTTGAGACAAG |
NGG |
2 |
0.4861 |
Tier III |
| 19 |
NC_000014.9 |
14 |
26909183 |
- |
MIR4307HG |
NNNCCATCTATTGAGAAAAG |
NGG |
2 |
0.1647 |
Tier III |
| 20 |
NC_000007.14 |
7 |
10478904 |
- |
MGC4859 |
NNNACATTTATTGAGTCAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
127548015 |
- |
Pard3 |
NNNACATTTATTGAGACAAG |
NGG |
1 |
0.875 |
Tier II |
| 2 |
NC_000071.6 |
5 |
49033323 |
- |
Kcnip4 |
NNNACATCTTTAGAGACAAG |
NGG |
2 |
0.7059 |
Tier II |
| 3 |
NC_000070.6 |
4 |
113780290 |
- |
Skint5 |
NNNACATTTACTGAGACAAG |
NGG |
2 |
0.5833 |
Tier II |
| 4 |
NC_000074.6 |
8 |
36899878 |
+ |
Dlc1 |
NNNACATCTATTCAGACAAA |
NGG |
2 |
0.3947 |
Tier II |
| 5 |
NC_000074.6 |
8 |
81533383 |
- |
Inpp4b |
NNNACATCTATTGAGAAAAT |
NGG |
2 |
0.3267 |
Tier II |
| 6 |
NC_000084.6 |
18 |
24656211 |
+ |
Mocos |
NNNACATGTATGGAGACAAG |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000070.6 |
4 |
151548098 |
- |
Camta1 |
NNNATATCTATTGAGATAAG |
NGG |
2 |
0.297 |
Tier II |
| 8 |
NC_000069.6 |
3 |
122155188 |
+ |
Abca4 |
NNNAAATCTATTTAGACAAG |
NGG |
2 |
0.1714 |
Tier II |
| 9 |
NC_000075.6 |
9 |
72087690 |
+ |
Tcf12 |
NNNACATCTATTGAGATAAG |
NAG |
2 |
0.121 |
Tier II |
| 10 |
NC_000080.6 |
14 |
75725737 |
+ |
Cog3 |
NNNCCATCTCTTGAGACAAG |
NGG |
2 |
0.1176 |
Tier II |
| 11 |
NC_000069.6 |
3 |
118743821 |
- |
Dpyd |
NNNACATCTCTTGAGGCAAG |
NGG |
2 |
0.0641 |
Tier II |
| 12 |
NC_000084.6 |
18 |
63120299 |
+ |
Piezo2 |
NNNACATCTATTGGGACAAG |
NGA |
2 |
0.0324 |
Tier II |
| 13 |
NC_000075.6 |
9 |
88398761 |
+ |
Snx14 |
NNNACATCTATTGAGAGTAG |
NGG |
2 |
0.0294 |
Tier II |
| 14 |
NC_000075.6 |
9 |
50872502 |
- |
Ppp2r1b |
NNNACATCTATTGAGGCAAG |
NGA |
2 |
0.0134 |
Tier II |
| 15 |
NC_000074.6 |
8 |
49400945 |
+ |
Tenm3 |
NNNACATCTATTGATACAAG |
NTG |
2 |
0.0056 |
Tier II |
| 16 |
NC_000070.6 |
4 |
7564089 |
+ |
8430436N08Rik |
NNNACATGTTTTGAGACAAG |
NGG |
2 |
0.5672 |
Tier III |
| 17 |
NC_000082.6 |
16 |
31482568 |
+ |
Gm34032 |
NNNACATTTATTGAGACCAG |
NGG |
2 |
0.1667 |
Tier III |
| 18 |
NC_000074.6 |
8 |
49400945 |
+ |
4930555F03Rik |
NNNACATCTATTGATACAAG |
NTG |
2 |
0.0056 |
Tier III |
Other clones with same target sequence:
(none)
