Construct: sgRNA BRDN0001145858

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACATCAAGTTCAAAGAACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNE (10020)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77830

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 36249245 + GNE NNNTCAAGTTCAAAGAACTC NGG 0 1.0 Tier I
2 NC_000023.11 X 107602951 + FRMPD3 NNNTCAAGTTCAAGGAGCTC NGG 2 0.0824 Tier I
3 NC_000021.9 21 46599381 + S100B NNNTCATGTTCAAAGAACTC NTG 2 0.017 Tier I
4 NC_000005.10 5 168918800 + SLIT3 NNNTCTAGCTCAAAGAACTC NGG 2 0.6593 Tier II
5 NC_000003.12 3 85164535 - CADM2 NNNTGAAATTCAAAGAACTC NGG 2 0.6 Tier II
6 NC_000020.11 20 34849093 - GGT7 NNNGCAAGTACAAAGAACTC NGG 2 0.5357 Tier II
7 NC_000001.11 1 224135271 - FBXO28 NNNCTAAGTTCAAAGAACTC NGG 2 0.4118 Tier II
8 NC_000009.12 9 105532870 - FSD1L NNNACAATTTCAAAGAACTC NGG 2 0.2976 Tier II
9 NC_000012.12 12 122543493 - KNTC1 NNNTCTAGTTCAAAGAACAC NGG 2 0.2041 Tier II
10 NC_000001.11 1 24178567 + IFNLR1 NNNTCAAGTTAAAAGGACTC NGG 2 0.1442 Tier II
11 NC_000007.14 7 42020296 + GLI3 NNNTCAAGTTCAAATAACTC NGG 1 0.1429 Tier II
12 NC_000007.14 7 84257298 - SEMA3A NNNACAAGTTCAAAGAACTC NAG 2 0.1235 Tier II
13 NC_000012.12 12 120716386 - UNC119B NNNTCAAGTTCAGAGAAGTC NGG 2 0.087 Tier II
14 NC_000011.10 11 133009475 + OPCML NNNTCAAGTTCAAAGAACCT NGG 2 0.0828 Tier II
15 NC_000008.11 8 123985493 + FER1L6 NNNTCAAGTTCAAAGAGCAC NGG 2 0.0504 Tier II
16 NC_000009.12 9 4830105 + RCL1 NNNTCAAGTTCTAAGAAGTC NGG 2 0.0444 Tier II
17 NC_000003.12 3 33712612 + CLASP2 NNNTCAAGTTCAAAGAAATG NGG 2 0.0317 Tier II
18 NC_000007.14 7 103159361 - LOC105375434 NNNTCAAGTTCACAGAAGTC NGG 2 0.0281 Tier II
19 NC_000018.10 18 79206753 - ATP9B NNNACAAGTTCAAAGAACTC NTG 2 0.0186 Tier II
20 NC_000005.10 5 133407011 + FSTL4 NNNTCAAGTTCAAGGAACTC NTG 2 0.0182 Tier II
21 NC_000019.10 19 6039792 + RFX2 NNNTGAAGTTCAAAGAACTC NGC 2 0.0133 Tier II
22 NC_000004.12 4 54722465 + KIT NNNTCAAGTTCAAAGAACTG NGA 2 0.0041 Tier II
23 NC_000009.12 9 84784012 + NTRK2 NNNTCAAGTTCAAATAACTC NGT 2 0.0023 Tier II
24 NC_000005.10 5 126919192 + MARCHF3 NNNTCAAGTTCAAAGCACTC NGA 2 0.0 Tier II
25 NC_000012.12 12 85407273 - LINC02820 NNNTTAAGTTCAAAGAATTC NGG 2 0.4091 Tier III
26 NC_000001.11 1 116430085 + LINC01762 NNNTCAAGGTCAGAGAACTC NGG 2 0.4037 Tier III
27 NC_000010.11 10 36926489 + LOC105376496 NNNACACGTTCAAAGAACTC NGG 2 0.2083 Tier III
28 NC_000008.11 8 123985493 + FER1L6-AS1 NNNTCAAGTTCAAAGAGCAC NGG 2 0.0504 Tier III
29 NC_000002.12 2 221738243 + LOC105373899 NNNTCAAGGTCAAAGAACTC NGA 2 0.043 Tier III
30 NC_000017.11 17 41353062 - LOC105371778 NNNTCAAGTTCAAACAAGTC NGG 2 0.0364 Tier III
31 NC_000005.10 5 15239948 - LINC02149 NNNTCAAGTTCAAAGATCTC NAG 2 0.0346 Tier III
32 NC_000005.10 5 130373106 + LOC105379171 NNNTCAAGTTCAAAGGCCTC NGG 2 0.0339 Tier III
33 NC_000003.12 3 107911777 + LINC00636 NNNTCAAGTTCACAGAACTG NGG 2 0.0124 Tier III
34 NC_000005.10 5 95872034 + LOC105379091 NNNTAAAGTTCAAAGAACTC NGT 2 0.0092 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 19694079 - Vmn2r102 NNNTGAAGTTCAAAGAACAC NGG 2 0.1714 Tier I
2 NC_000083.6 17 19811870 - Vmn2r103 NNNTGAAGTTCAAAGAACAC NGG 2 0.1714 Tier I
3 NC_000068.7 2 76965102 - Ttn NNNTCAAGTTCAAATAACTC NGG 1 0.1429 Tier I
4 NC_000076.6 10 4357371 - Akap12 NNNTCAAGTTCCAAGATCTC NGG 2 0.0351 Tier I
5 NC_000076.6 10 76260051 - S100b NNNTCATGTTCAAAGAACTC NTG 2 0.017 Tier I
6 NC_000081.6 15 10181119 + Prlr NNNTCAAGTTCAAAGAACTC NGG 0 1.0 Tier II
7 NC_000075.6 9 43228719 - Oaf NNNTCAAGATCAGAGAACTC NGG 2 0.6056 Tier II
8 NC_000072.6 6 22198284 + Cped1 NNNTGAAGATCAAAGAACTC NGG 2 0.5571 Tier II
9 NC_000070.6 4 100354224 - Ror1 NNNCCAATTTCAAAGAACTC NGG 2 0.4044 Tier II
10 NC_000081.6 15 22780544 - Cdh18 NNNTAAACTTCAAAGAACTC NGG 2 0.3516 Tier II
11 NC_000068.7 2 127729479 - Mall NNNTAAAGTTCAAAGAACTA NGG 2 0.2857 Tier II
12 NC_000073.6 7 44763712 - Vrk3 NNNTCTAGTTCAAAGAACCC NGG 2 0.197 Tier II
13 NC_000074.6 8 61814089 + Palld NNNTCAAGTTCAGAGAACAC NGG 2 0.1863 Tier II
14 NC_000075.6 9 15601903 + Deup1 NNNTCAAGTTCTATGAACTC NGG 2 0.1778 Tier II
15 NC_000071.6 5 108537255 - Cplx1 NNNTTAAGTTCCAAGAACTC NGG 2 0.1675 Tier II
16 NC_000067.6 1 11104460 + Prex2 NNNTTAAGTTCAAAGAACTC NAG 2 0.165 Tier II
17 NC_000078.6 12 5111671 - Klhl29 NNNTCAAGTTCATGGAACTC NGG 2 0.14 Tier II
18 NC_000069.6 3 105755223 + Rap1a NNNTCAAGTTCAAACAACTA NGG 2 0.1364 Tier II
19 NC_000069.6 3 89753051 - Adar NNNTCAAGTCCACAGAACTC NGG 2 0.1123 Tier II
20 NC_000075.6 9 97835108 + Clstn2 NNNTCAAGTTCAAAGAACTT NAG 2 0.0778 Tier II
21 NC_000080.6 14 67052816 - Ppp2r2a NNNTCAAGTTTACAGAACTC NGG 2 0.0648 Tier II
22 NC_000075.6 9 108367302 + Usp4 NNNTCAAGTTCAAAGATCTT NGG 2 0.04 Tier II
23 NC_000074.6 8 94749260 + Ccl22 NNNTCAAGTTCAAGGAACTG NGG 2 0.0275 Tier II
24 NC_000067.6 1 38186631 + Aff3 NNNTCACGTTCAAAGAACTG NGG 2 0.0257 Tier II
25 NC_000079.6 13 52840166 - Auh NNNTCAAGATCAAAGAACTC NGC 2 0.0206 Tier II
26 NC_000076.6 10 128648554 - Ikzf4 NNNTCAAGTTAAAAGAACTC NGC 2 0.0167 Tier II
27 NC_000072.6 6 28274331 + Zfp800 NNNTCAAGTTCATAGAACTC NGT 2 0.0048 Tier II
28 NC_000069.6 3 83944298 + Tmem131l NNNTCAAGTTCAAAGATCTC NGC 2 0.003 Tier II
29 NC_000075.6 9 21005719 - Mrpl4 NNNTCAAGCTCAAAGTACTC NGG 2 0.0 Tier II
30 NC_000073.6 7 19349613 - Ercc1 NNNTCAAGGTCAAAGCACTC NGG 2 0.0 Tier II
31 NC_000080.6 14 22375192 - Lrmda NNNTCAAGTTCAAAGTCCTC NGG 2 0.0 Tier II
32 NC_000079.6 13 89503902 + Gm34699 NNNTCAATTTCAAAGAATTC NGG 2 0.4018 Tier III
33 NC_000083.6 17 19303974 - Vmn2r-ps120 NNNTGAAGTTCAAAGAACAC NGG 2 0.1714 Tier III
34 NC_000083.6 17 19727399 - Vmn2r-ps122 NNNTGAAGTTCAAAGAACAC NGG 2 0.1714 Tier III
35 NC_000069.6 3 149213577 - Gm30382 NNNTCAAGTTCCCAGAACTC NGG 2 0.0554 Tier III
36 NC_000079.6 13 52995166 + Gm33424 NNNTCAAGTTCAAAGAACTT NGA 2 0.0208 Tier III
37 NC_000079.6 13 52840166 - Gm33315 NNNTCAAGATCAAAGAACTC NGC 2 0.0206 Tier III
38 NC_000076.6 10 128648554 - Gm26876 NNNTCAAGTTAAAAGAACTC NGC 2 0.0167 Tier III
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Other clones with same target sequence:

(none)