Construct: sgRNA BRDN0001145863
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTAAGGCGTATCTCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB2 (5208)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77685
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
207063187 |
+ |
PFKFB2 |
NNNTAAGGCGTATCTCACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
58496538 |
+ |
DAB1 |
NNNTAAAGCTTATCTCACTG |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000001.11 |
1 |
58496538 |
+ |
OMA1 |
NNNTAAAGCTTATCTCACTG |
NGG |
2 |
0.8125 |
Tier II |
| 4 |
NC_000008.11 |
8 |
103590772 |
+ |
RIMS2 |
NNNTAAGGCATATTTCACTG |
NGG |
2 |
0.6844 |
Tier II |
| 5 |
NC_000022.11 |
22 |
40412271 |
- |
MRTFA |
NNNTGAGGTGTATCTCACTG |
NGG |
2 |
0.63 |
Tier II |
| 6 |
NC_000019.10 |
19 |
46045921 |
- |
IGFL4 |
NNNTATGGAGTATCTCACTG |
NGG |
2 |
0.6122 |
Tier II |
| 7 |
NC_000002.12 |
2 |
182040370 |
+ |
PPP1R1C |
NNNTAAGGTGTATCTCATTG |
NGG |
2 |
0.5625 |
Tier II |
| 8 |
NC_000016.10 |
16 |
5311616 |
+ |
RBFOX1 |
NNNTAAGGTGTATCTCATTG |
NGG |
2 |
0.5625 |
Tier II |
| 9 |
NC_000020.11 |
20 |
20253418 |
+ |
CFAP61 |
NNNTAAGGCTCATCTCACTG |
NGG |
2 |
0.5417 |
Tier II |
| 10 |
NC_000003.12 |
3 |
130693515 |
+ |
PIK3R4 |
NNNGAAGGAGTATCTCACTG |
NGG |
2 |
0.5357 |
Tier II |
| 11 |
NC_000013.11 |
13 |
49675930 |
+ |
EBPL |
NNNGAAGGAGTATCTCACTG |
NGG |
2 |
0.5357 |
Tier II |
| 12 |
NC_000019.10 |
19 |
15916719 |
- |
CYP4F11 |
NNNTAGGGGGTATCTCACTG |
NGG |
2 |
0.4422 |
Tier II |
| 13 |
NC_000010.11 |
10 |
89627979 |
- |
PANK1 |
NNNTAAGTCGTATCTCATTG |
NGG |
2 |
0.4018 |
Tier II |
| 14 |
NC_000005.10 |
5 |
76686018 |
+ |
IQGAP2 |
NNNGAAGTCGTATCTCACTG |
NGG |
2 |
0.3906 |
Tier II |
| 15 |
NC_000015.10 |
15 |
74663872 |
- |
EDC3 |
NNNTAAGGCGTCTCCCACTG |
NGG |
2 |
0.0718 |
Tier II |
| 16 |
NC_000016.10 |
16 |
12956177 |
- |
SHISA9 |
NNNTAAGGCGTAGCTCAGTG |
NGG |
2 |
0.0348 |
Tier II |
| 17 |
NC_000005.10 |
5 |
135282641 |
+ |
PITX1-AS1 |
NNNTGAGGTGTATCTCACTG |
NGG |
2 |
0.63 |
Tier III |
| 18 |
NC_000020.11 |
20 |
20253418 |
+ |
RPL17P1 |
NNNTAAGGCTCATCTCACTG |
NGG |
2 |
0.5417 |
Tier III |
| 19 |
NC_000001.11 |
1 |
224815291 |
- |
LOC105373108 |
NNNTAAGTAGTATCTCACTG |
NGG |
2 |
0.5357 |
Tier III |
| 20 |
NC_000020.11 |
20 |
39786142 |
- |
LOC105372613 |
NNNGAAGGGGTATCTCACTG |
NGG |
2 |
0.3869 |
Tier III |
| 21 |
NC_000020.11 |
20 |
39786142 |
- |
LOC105372614 |
NNNGAAGGGGTATCTCACTG |
NGG |
2 |
0.3869 |
Tier III |
| 22 |
NC_000007.14 |
7 |
156502790 |
+ |
RNF32-DT |
NNNTAAGGAGTATCTCACAG |
NGG |
2 |
0.2449 |
Tier III |
| 23 |
NC_000010.11 |
10 |
131866847 |
- |
LOC105378565 |
NNNTGAGGCGTATCTCAGTG |
NGG |
2 |
0.096 |
Tier III |
| 24 |
NC_000016.10 |
16 |
12956177 |
- |
LOC105371092 |
NNNTAAGGCGTAGCTCAGTG |
NGG |
2 |
0.0348 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
117738739 |
- |
Slc35e3 |
NNNTAAGGAGTATCTCACTG |
NGG |
1 |
0.8571 |
Tier II |
| 2 |
NC_000076.6 |
10 |
40038939 |
+ |
Slc16a10 |
NNNTAAGGTGAATCTCACTG |
NGG |
2 |
0.6563 |
Tier II |
| 3 |
NC_000072.6 |
6 |
145024687 |
- |
Bcat1 |
NNNTAAGGCATATCTCACTT |
NGG |
2 |
0.6533 |
Tier II |
| 4 |
NC_000069.6 |
3 |
108032718 |
- |
Gstm4 |
NNNGAAGGTGTATCTCACTG |
NGG |
2 |
0.5469 |
Tier II |
| 5 |
NC_000079.6 |
13 |
108337473 |
+ |
Depdc1b |
NNNTAGGGCGTGTCTCACTG |
NGG |
2 |
0.5159 |
Tier II |
| 6 |
NC_000076.6 |
10 |
36705063 |
- |
Hs3st5 |
NNNGAAGGGGTATCTCACTG |
NGG |
2 |
0.3869 |
Tier II |
| 7 |
NC_000071.6 |
5 |
121768514 |
- |
Atxn2 |
NNNAAAGGCCTATCTCACTG |
NGG |
2 |
0.1905 |
Tier II |
| 8 |
NC_000069.6 |
3 |
108032718 |
- |
Gm36498 |
NNNGAAGGTGTATCTCACTG |
NGG |
2 |
0.5469 |
Tier III |
Other clones with same target sequence:
(none)
