Construct: sgRNA BRDN0001145865
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAACATCGTCAAAGTGTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK4 (5596)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77222
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
50664200 |
+ |
MAPK4 |
NNNCATCGTCAAAGTGTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
64826708 |
+ |
SPTB |
NNNCCTCGTCAAAGTGTAGG |
NGG |
2 |
0.0625 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
73970196 |
- |
Mapk4 |
NNNCATTGTCAAGGTGTACG |
NGG |
2 |
0.5299 |
Tier I |
| 2 |
NC_000085.6 |
19 |
41296378 |
- |
Pik3ap1 |
NNNCATCGTCAACGTGGACG |
NGG |
2 |
0.0248 |
Tier I |
| 3 |
NC_000071.6 |
5 |
19927539 |
- |
Magi2 |
NNNCATCTTCAAAATGTACG |
NGG |
2 |
0.4688 |
Tier II |
| 4 |
NC_000070.6 |
4 |
4402837 |
- |
Gm33007 |
NNNCATGGACAAAGTGTACG |
NGG |
2 |
0.437 |
Tier III |
| 5 |
NC_000067.6 |
1 |
172770900 |
+ |
Gm38051 |
NNNCATGGTCAAAGTGGACG |
NGG |
2 |
0.0554 |
Tier III |
Other clones with same target sequence:
(none)
