Construct: sgRNA BRDN0001145867
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGAGCCAAACTCGCCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPHK2 (56848)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77094
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
48626007 |
- |
SPHK2 |
NNNAGCCAAACTCGCCATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
8369220 |
- |
ACOX3 |
NNNAGCCAGACCCGCCATGG |
NGG |
2 |
0.6316 |
Tier II |
| 3 |
NC_000022.11 |
22 |
39573608 |
+ |
CACNA1I |
NNNAGCCACACACGCCATGG |
NGG |
2 |
0.4571 |
Tier II |
| 4 |
NC_000013.11 |
13 |
23632816 |
+ |
TNFRSF19 |
NNNAGCCAAACCAGCCATGG |
NGG |
2 |
0.3644 |
Tier II |
| 5 |
NC_000002.12 |
2 |
227034011 |
- |
COL4A4 |
NNNAGCCACACTCGCCATGC |
NGG |
2 |
0.2449 |
Tier II |
| 6 |
NC_000001.11 |
1 |
54594095 |
- |
ACOT11 |
NNNAGCCAGACTCGCCATGG |
NAG |
2 |
0.1728 |
Tier II |
| 7 |
NC_000020.11 |
20 |
43689383 |
- |
MYBL2 |
NNNAGCCAAACAGGCCATGG |
NGG |
2 |
0.1091 |
Tier II |
| 8 |
NC_000009.12 |
9 |
760146 |
- |
LOC124902109 |
NNNGGCCAAACCCGCCATGG |
NGG |
2 |
0.5921 |
Tier III |
| 9 |
NC_000024.10 |
Y |
15282822 |
- |
LOC107987355 |
NNNAGCCAAATTCACCATGG |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
77836000 |
+ |
Krtap10-10 |
NNNAGCCAAACCCGCCATGG |
NCG |
2 |
0.1015 |
Tier I |
| 2 |
NC_000076.6 |
10 |
77836000 |
+ |
Tspear |
NNNAGCCAAACCCGCCATGG |
NCG |
2 |
0.1015 |
Tier II |
| 3 |
NC_000076.6 |
10 |
79953111 |
+ |
Arid3a |
NNNAGCCAAACTAGGCATGG |
NGG |
2 |
0.0192 |
Tier II |
| 4 |
NC_000081.6 |
15 |
85706054 |
- |
Lncppara |
NNNAGCCTAACTCCCCATGG |
NGG |
2 |
0.1837 |
Tier III |
| 5 |
NC_000068.7 |
2 |
162671527 |
+ |
9430021M05Rik |
NNNATCCATACTCGCCATGG |
NGG |
2 |
0.18 |
Tier III |
Other clones with same target sequence:
(none)
