Construct: sgRNA BRDN0001145868
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGGAAATGATCCGCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MTOR (2475)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76784
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11238587 |
+ |
MTOR |
NNNGGAAATGATCCGCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
72342085 |
- |
RIMS1 |
NNNGGAAAGGATCTGCACAG |
NGG |
2 |
0.454 |
Tier II |
| 3 |
NC_000006.12 |
6 |
96835429 |
- |
GPR63 |
NNNGGAAATGATACACACAG |
NGG |
2 |
0.362 |
Tier II |
| 4 |
NC_000020.11 |
20 |
43093861 |
- |
PTPRT |
NNNGGAAATGGTCAGCACAG |
NGG |
2 |
0.2275 |
Tier II |
| 5 |
NC_000015.10 |
15 |
59655159 |
- |
GTF2A2 |
NNNGGAAATGATCCGCAGAG |
NGG |
1 |
0.1333 |
Tier II |
| 6 |
NC_000017.11 |
17 |
1177621 |
+ |
ABR |
NNNGGAAATGATCAGCTCAG |
NGG |
2 |
0.0467 |
Tier II |
| 7 |
NC_000006.12 |
6 |
50716250 |
+ |
TFAP2D |
NNNGGAAATAATCCGCACAG |
NGC |
2 |
0.0207 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
75494049 |
+ |
Obsl1 |
NNNGTATATGATCCGCACAG |
NGG |
2 |
0.1313 |
Tier I |
| 2 |
NC_000081.6 |
15 |
78292826 |
+ |
Csf2rb2 |
NNNGGAAATGATCCCCAGAG |
NGG |
2 |
0.0364 |
Tier I |
| 3 |
NC_000085.6 |
19 |
56584523 |
- |
Nhlrc2 |
NNNGGAAAGAATCCGCACAG |
NGG |
2 |
0.5778 |
Tier II |
| 4 |
NC_000079.6 |
13 |
100030165 |
+ |
Bdp1 |
NNNGGAAATGTTCCTCACAG |
NGG |
2 |
0.044 |
Tier II |
| 5 |
NC_000077.6 |
11 |
36362646 |
- |
Tenm2 |
NNNGGAAATGATCCTCACAG |
NGT |
2 |
0.0023 |
Tier II |
| 6 |
NC_000068.7 |
2 |
10166589 |
+ |
Itih5 |
NNNGGAAATGATCGGCACGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000077.6 |
11 |
36362646 |
- |
Gm33585 |
NNNGGAAATGATCCTCACAG |
NGT |
2 |
0.0023 |
Tier III |
Other clones with same target sequence:
(none)
