Construct: sgRNA BRDN0001145869

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCATGCTTGCTTGGGCAAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80256

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 53776015 + FTO NNNTACTTGCTTGGGAAAAA NGG 2 0.8667 Tier II
2 NC_000014.9 14 78949878 + NRXN3 NNNTGCATGCTAGGGCAAAA NGG 2 0.7 Tier II
3 NC_000009.12 9 113289501 - PRPF4 NNNTGCTAGATTGGGCAAAA NGG 2 0.6933 Tier II
4 NC_000006.12 6 90574841 - MAP3K7 NNNTGCTTTCTTGAGCAAAA NGG 2 0.4 Tier II
5 NC_000014.9 14 77225837 - TMEM63C NNNTGTTTGCTTGGGCAGAA NGG 2 0.3714 Tier II
6 NC_000007.14 7 43242403 + HECW1 NNNTGCTTGGTTGGACAAAA NGG 2 0.366 Tier II
7 NC_000016.10 16 81346950 - GAN NNNTGCTTGCTTTGGAAAAA NGG 2 0.3 Tier II
8 NC_000005.10 5 59324163 - PDE4D NNNTGCTTGCTTGAGCAAGA NGG 2 0.2813 Tier II
9 NC_000019.10 19 15237778 - BRD4 NNNTGTTTGCTTGTGCAAAA NGG 2 0.2476 Tier II
10 NC_000009.12 9 121765075 + DAB2IP NNNTGCTTGCCTGGGCCAAA NGG 2 0.1176 Tier II
11 NC_000015.10 15 100512231 + CERS3 NNNTGCCTGCTTGGGGAAAA NGG 2 0.1058 Tier II
12 NC_000002.12 2 24829922 - ADCY3 NNNTGCTTTCTTGGGGAAAA NGG 2 0.0821 Tier II
13 NC_000019.10 19 6757672 + SH2D3A NNNTGCTTGCTTGGGTACAA NGG 2 0.0586 Tier II
14 NC_000003.12 3 122011981 - ILDR1 NNNTGCTTGCTTGGGAAAAA NTG 2 0.039 Tier II
15 NC_000007.14 7 90698717 + CDK14 NNNTGCTTGCTTTGGCAAAA NTG 2 0.0117 Tier II
16 NC_000020.11 20 41126246 - PLCG1-AS1 NNNTGCTTGTTTGGGAAAAA NGG 2 0.9412 Tier III
17 NC_000017.11 17 72535230 - LINC00673 NNNTGCTTGCGTGGGCAAAA NGG 1 0.4 Tier III
18 NC_000023.11 X 145057250 + RN7SKP189 NNNTGCTTGCTTGGTCAAAG NGG 2 0.1092 Tier III
19 NC_000013.11 13 97573184 + LOC105370324 NNNTGCTTGCTTGGCTAAAA NGG 2 0.0839 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 22986496 + Abi1 NNNTGCTTGCTTAGGCAAAT NGG 2 0.5538 Tier II
2 NC_000071.6 5 141522732 + Sdk1 NNNGACTTGCTTGGGCAAAA NGG 2 0.5417 Tier II
3 NC_000073.6 7 78535809 - Ntrk3 NNNTGCTAGCTTGGGCAGAA NGG 2 0.32 Tier II
4 NC_000078.6 12 111864256 - Ppp1r13b NNNTGCTAGCTTGGGCAGAA NGG 2 0.32 Tier II
5 NC_000072.6 6 73058076 - Dnah6 NNNTGCTTGCTAGGGCAGAA NGG 2 0.32 Tier II
6 NC_000067.6 1 175330206 + Rgs7 NNNTGCTTGCTTGGGAACAA NGG 2 0.1905 Tier II
7 NC_000079.6 13 68589067 - Fastkd3 NNNTGCTTCCTTGTGCAAAA NGG 2 0.1436 Tier II
8 NC_000085.6 19 46047952 - Ldb1 NNNTGCTTGCTTGTGCAACA NGG 2 0.0552 Tier II
9 NC_000071.6 5 141779067 + Sdk1 NNNTGCTTGCTTGGGCAAAT NGT 2 0.0097 Tier II
10 NC_000068.7 2 102087754 - Ldlrad3 NNNTGCTTGCTTTGGCAAAA NGT 2 0.0048 Tier II
11 NC_000080.6 14 76042305 + Gm36477 NNNTGTTTGCTTGGGCAGAA NGG 2 0.3714 Tier III
12 NC_000084.6 18 77793660 + F830208F22Rik NNNTGCTTGCTTTGGCATAA NGG 2 0.15 Tier III
13 NC_000069.6 3 144894621 + Clca4c-ps NNNTGCCTGCTTGGGCAAAA NTG 2 0.0268 Tier III
14 NC_000070.6 4 82071795 + Gm5860 NNNTGCTTGCTTGGCCAAAA NGA 2 0.0189 Tier III
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Other clones with same target sequence:

(none)