Construct: sgRNA BRDN0001145870
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATAAAATACATTGTCAACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK39 (27347)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76071
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
168163847 |
- |
STK39 |
NNNAAATACATTGTCAACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
47035194 |
+ |
CYP4X1 |
NNNAAATACATTTTCAACCA |
NGG |
2 |
0.2813 |
Tier II |
| 3 |
NC_000001.11 |
1 |
197052577 |
+ |
F13B |
NNNAAATAAATTTTCAACCG |
NGG |
2 |
0.2571 |
Tier II |
| 4 |
NC_000002.12 |
2 |
40246277 |
+ |
SLC8A1 |
NNNAAATACATTCTCAACAG |
NGG |
2 |
0.1805 |
Tier II |
| 5 |
NC_000001.11 |
1 |
24320763 |
- |
GRHL3 |
NNNCAATACATTGTCAACCC |
NGG |
2 |
0.1513 |
Tier II |
| 6 |
NC_000006.12 |
6 |
84174646 |
- |
CEP162 |
NNNAAATACATTGTCATCCT |
NGG |
2 |
0.0933 |
Tier II |
| 7 |
NC_000001.11 |
1 |
196321878 |
+ |
KCNT2 |
NNNAAATACATTGTCAACAG |
NGT |
2 |
0.0069 |
Tier II |
| 8 |
NC_000003.12 |
3 |
8297078 |
+ |
LMCD1-AS1 |
NNNAAAGGCATTGTCAACCG |
NGG |
2 |
0.4314 |
Tier III |
| 9 |
NC_000002.12 |
2 |
40246277 |
+ |
SLC8A1-AS1 |
NNNAAATACATTCTCAACAG |
NGG |
2 |
0.1805 |
Tier III |
| 10 |
NC_000010.11 |
10 |
3739901 |
+ |
LOC105376360 |
NNNAAATACATTTTCAAACG |
NGG |
2 |
0.1615 |
Tier III |
| 11 |
NC_000001.11 |
1 |
24320763 |
- |
GRHL3-AS1 |
NNNCAATACATTGTCAACCC |
NGG |
2 |
0.1513 |
Tier III |
| 12 |
NC_000001.11 |
1 |
246686749 |
- |
LOC102724382 |
NNNAAATACATTGTCAAGGG |
NGG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
145805025 |
- |
Rin2 |
NNNAAATACACTGTCAACTG |
NGG |
2 |
0.3077 |
Tier II |
| 2 |
NC_000067.6 |
1 |
9088367 |
+ |
Sntg1 |
NNNAAATACATTTTCAACCA |
NGG |
2 |
0.2813 |
Tier II |
| 3 |
NC_000071.6 |
5 |
36491846 |
+ |
Tbc1d14 |
NNNAAATACATTCTCAACCC |
NGG |
2 |
0.1805 |
Tier II |
| 4 |
NC_000081.6 |
15 |
35748611 |
+ |
Vps13b |
NNNAAATCCATTCTCAACCG |
NGG |
2 |
0.1805 |
Tier II |
| 5 |
NC_000082.6 |
16 |
66875099 |
+ |
Cadm2 |
NNNAAATACATTATCAACCG |
NGT |
2 |
0.0149 |
Tier II |
| 6 |
NC_000074.6 |
8 |
82809430 |
+ |
Gm31545 |
NNNAAATACATGGTCAACTG |
NGG |
2 |
0.2308 |
Tier III |
| 7 |
NC_000067.6 |
1 |
101162974 |
- |
Gm20268 |
NNNAAATACATTGTCAACAG |
NAG |
2 |
0.1111 |
Tier III |
Other clones with same target sequence:
(none)
