Construct: sgRNA BRDN0001145875
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTATACATCCAAGCTCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PLXNA4 (91584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76880
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
132507854 |
- |
PLXNA4 |
NNNATACATCCAAGCTCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
41894590 |
+ |
C2CD2 |
NNNATACATCAAAGCCCGTG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000008.11 |
8 |
10919069 |
+ |
XKR6 |
NNNATAAATCCAAGCTCCTG |
NGG |
2 |
0.3571 |
Tier II |
| 4 |
NC_000005.10 |
5 |
132545629 |
+ |
IL5 |
NNNATACATCAAAGCTGGTG |
NGG |
2 |
0.0441 |
Tier II |
| 5 |
NC_000003.12 |
3 |
178568360 |
- |
KCNMB2 |
NNNATACATCCAAGCTGGTG |
NAG |
2 |
0.0153 |
Tier II |
| 6 |
NC_000005.10 |
5 |
117915609 |
+ |
LINC02147 |
NNNATATATCCAAGCTCTTG |
NGG |
2 |
0.5417 |
Tier III |
| 7 |
NC_000020.11 |
20 |
39168573 |
- |
LOC107985448 |
NNNATTCATCCAAGCTCTTG |
NGG |
2 |
0.4762 |
Tier III |
| 8 |
NC_000003.12 |
3 |
178568360 |
- |
KCNMB2-AS1 |
NNNATACATCCAAGCTGGTG |
NAG |
2 |
0.0153 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
64510400 |
- |
Apba2 |
NNNAAACATCCAAGCTCATG |
NGG |
2 |
0.3462 |
Tier II |
| 2 |
NC_000069.6 |
3 |
30914077 |
+ |
Phc3 |
NNNAAACATCCAGGCTCGTG |
NGG |
2 |
0.3261 |
Tier II |
| 3 |
NC_000083.6 |
17 |
67087265 |
- |
Ptprm |
NNNATGCATCCAATCTCGTG |
NGG |
2 |
0.1905 |
Tier II |
| 4 |
NC_000080.6 |
14 |
62666658 |
+ |
Serpine3 |
NNNATACATCCAAGCTGGTC |
NGG |
2 |
0.0252 |
Tier II |
| 5 |
NC_000067.6 |
1 |
61391916 |
+ |
9530026F06Rik |
NNNATACATCCAAGCACCTG |
NGG |
2 |
0.4329 |
Tier III |
Other clones with same target sequence:
(none)
