Construct: sgRNA BRDN0001145876

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAATAACATCACCATGCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYN (4067)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77027

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 55952003 - LYN NNNTAACATCACCATGCACA NGG 0 1.0 Tier I
2 NC_000021.9 21 5045014 + LOC102723996 NNNTAACAACACCATGCACA NGG 1 0.9286 Tier II
3 NC_000021.9 21 44218650 - ICOSLG NNNTAACAACACCATGCACA NGG 1 0.9286 Tier II
4 NC_000003.12 3 28252720 - CMC1 NNNTGACACCACCATGCACA NGG 2 0.6646 Tier II
5 NC_000008.11 8 118387860 + SAMD12 NNNTAGCATCACTATGCACA NGG 2 0.5 Tier II
6 NC_000023.11 X 35931286 - CFAP47 NNNGAAAATCACCATGCACA NGG 2 0.4688 Tier II
7 NC_000011.10 11 108166634 + NPAT NNNTAACATTACCATGTACA NGG 2 0.4392 Tier II
8 NC_000001.11 1 213655876 - RPS6KC1 NNNTAACATCACAATACACA NGG 2 0.3846 Tier II
9 NC_000010.11 10 60304865 + ANK3 NNNTACAATCACCATGCACA NGG 2 0.3409 Tier II
10 NC_000003.12 3 23500599 + UBE2E2 NNNTAACGTCACAATGCACA NGG 2 0.2821 Tier II
11 NC_000011.10 11 41426433 + LRRC4C NNNAAACATCACCATGCAAA NGG 2 0.2041 Tier II
12 NC_000003.12 3 133804286 - SRPRB NNNAAACCTCACCATGCACA NGG 2 0.2041 Tier II
13 NC_000001.11 1 115290909 - NGF NNNTAACATCATCATGCAGA NGG 2 0.0673 Tier II
14 NC_000005.10 5 11584765 + CTNND2 NNNTAATATCACCATGCACA NGA 2 0.0564 Tier II
15 NC_000021.9 21 41228303 + BACE2 NNNTAACATCACCATGGACA NGT 2 0.0009 Tier II
16 NC_000007.14 7 32130768 + PDE1C NNNTAACATCACCATGCACA NCA 2 0.0 Tier II
17 NC_000011.10 11 11318426 - GALNT18 NNNTAACATCACCCTTCACA NGG 2 0.0 Tier II
18 NC_000008.11 8 118387860 + LOC105375724 NNNTAGCATCACTATGCACA NGG 2 0.5 Tier III
19 NC_000017.11 17 59542407 + LOC105371847 NNNTAACTTTACCATGCACA NGG 2 0.4034 Tier III
20 NC_000006.12 6 166241623 + LOC101929297 NNNTTACAGCACCATGCACA NGG 2 0.2251 Tier III
21 NC_000003.12 3 133804286 - LOC105374116 NNNAAACCTCACCATGCACA NGG 2 0.2041 Tier III
22 NC_000001.11 1 115290909 - NGF-AS1 NNNTAACATCATCATGCAGA NGG 2 0.0673 Tier III
23 NC_000020.11 20 52410560 + LOC105372666 NNNTCACATCACCATGCACA NTG 2 0.0195 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 3746748 - Lyn NNNTGACATCACCATGCATA NGG 2 0.3323 Tier I
2 NC_000073.6 7 19970270 + Ceacam20 NNNTAACATCACCATCCACT NGG 2 0.0 Tier I
3 NC_000071.6 5 115029532 + Sppl3 NNNTAGCATCAACATGCACA NGG 2 0.5102 Tier II
4 NC_000069.6 3 119752252 + Ptbp2 NNNCAGCATCACCATGCACA NGG 2 0.4622 Tier II
5 NC_000067.6 1 62167814 + Pard3b NNNTAACAACAGCATGCACA NGG 2 0.4127 Tier II
6 NC_000080.6 14 69790617 + Rhobtb2 NNNTAACTACACCATGCACA NGG 2 0.398 Tier II
7 NC_000068.7 2 76206937 - Pde11a NNNTAATATCACCATGCAAA NGG 2 0.3482 Tier II
8 NC_000079.6 13 112900100 + Mtrex NNNCAACATCACCATGCTCA NGG 2 0.3235 Tier II
9 NC_000071.6 5 124205127 + Pitpnm2 NNNTGACTTCACCATGCACA NGG 2 0.3086 Tier II
10 NC_000072.6 6 43618112 - Tpk1 NNNTAACAGCACCATGAACA NGG 2 0.2889 Tier II
11 NC_000085.6 19 37634719 - Exoc6 NNNTGACATCACAATGCACA NGG 2 0.2769 Tier II
12 NC_000071.6 5 74221838 + Scfd2 NNNAAACATCAGCATGCACA NGG 2 0.2116 Tier II
13 NC_000075.6 9 116894649 + Rbms3 NNNTAACATCACAATGAACA NGG 2 0.1795 Tier II
14 NC_000070.6 4 62637529 - Rgs3 NNNTAACATCACCATGCTCA NAG 2 0.1296 Tier II
15 NC_000078.6 12 73632729 - Prkch NNNTAACACCACCATGCACA NGA 2 0.0641 Tier II
16 NC_000082.6 16 57424683 + Cmss1 NNNTAACATCAACATGCACA NGA 2 0.0496 Tier II
17 NC_000082.6 16 57424683 + Filip1l NNNTAACATCAACATGCACA NGA 2 0.0496 Tier II
18 NC_000070.6 4 66029175 - Astn2 NNNCAACATCACCATGCACA NTG 2 0.0252 Tier II
19 NC_000086.7 X 164099497 - Cltrn NNNTAACAGCACCATGCACA NGC 2 0.0138 Tier II
20 NC_000068.7 2 140412906 + Macrod2 NNNTAACATCACCACTCACA NGG 2 0.0 Tier II
21 NC_000072.6 6 66207995 - Gm36408 NNNTAACATCATCATACACA NGG 2 0.5385 Tier III
22 NC_000086.7 X 91557567 + Gm46677 NNNAAACATCACTATGCACA NGG 2 0.3333 Tier III
23 NC_000070.6 4 3508161 + Gm11787 NNNTGACATCACCATGCATA NGG 2 0.3323 Tier III
24 NC_000076.6 10 19172655 + Gm20139 NNNTAACATCAGTATGCACA NGG 2 0.3111 Tier III
25 NC_000069.6 3 28186139 + Gm42196 NNNTTACATCACCATGCCCA NGG 2 0.0693 Tier III
26 NC_000071.6 5 23876374 - Gm9534 NNNTACCATCACCATCCACA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)