Construct: sgRNA BRDN0001145877
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGAGAACTCGAGTTTGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACA (5566)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75517
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
14102838 |
+ |
PRKACA |
NNNAGAACTCGAGTTTGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
31276983 |
+ |
LIMK2 |
NNNAGAACTAGAGATTGACG |
NGG |
2 |
0.5365 |
Tier I |
| 3 |
NC_000011.10 |
11 |
64245260 |
- |
PPP1R14B |
NNNAGAACTGGAGATTGACG |
NGG |
2 |
0.2407 |
Tier I |
| 4 |
NC_000009.12 |
9 |
69013779 |
+ |
PRKACG |
NNNAGAACTGGAGCTTGACG |
NGG |
2 |
0.1111 |
Tier I |
| 5 |
NC_000009.12 |
9 |
133823815 |
+ |
VAV2 |
NNNAGCACTCGAGTTTAACG |
NGG |
2 |
0.4242 |
Tier II |
| 6 |
NC_000002.12 |
2 |
33195516 |
+ |
LTBP1 |
NNNAGAACTCGAGTTAGAAG |
NGG |
2 |
0.3896 |
Tier II |
| 7 |
NC_000007.14 |
7 |
12378876 |
- |
VWDE |
NNNAGGACTCGAGTTTGACC |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000004.12 |
4 |
139115088 |
- |
ELF2 |
NNNAGAACTGGAGATTGACG |
NGG |
2 |
0.2407 |
Tier II |
| 9 |
NC_000006.12 |
6 |
126258079 |
- |
TRMT11 |
NNNAGAACTGGAGATTGACG |
NGG |
2 |
0.2407 |
Tier II |
| 10 |
NC_000013.11 |
13 |
110650819 |
+ |
CARS2 |
NNNAGGACTCGAGCTTGACG |
NGG |
2 |
0.2041 |
Tier II |
| 11 |
NC_000022.11 |
22 |
31276983 |
+ |
PPP1R14BP1 |
NNNAGAACTAGAGATTGACG |
NGG |
2 |
0.5365 |
Tier III |
| 12 |
NC_000004.12 |
4 |
139115088 |
- |
PPP1R14BP3 |
NNNAGAACTGGAGATTGACG |
NGG |
2 |
0.2407 |
Tier III |
| 13 |
NC_000006.12 |
6 |
126258079 |
- |
PPP1R14BP5 |
NNNAGAACTGGAGATTGACG |
NGG |
2 |
0.2407 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
20620220 |
+ |
Etl4 |
NNNAGAACTCGAGTCTGAAG |
NGG |
2 |
0.1169 |
Tier II |
| 2 |
NC_000074.6 |
8 |
108724145 |
+ |
Zfhx3 |
NNNTGAACTCGAGTTTGAGG |
NGG |
2 |
0.0795 |
Tier II |
| 3 |
NC_000067.6 |
1 |
181836782 |
- |
Lbr |
NNNAGAACTCGAGTTTGAGC |
NGG |
2 |
0.0536 |
Tier II |
| 4 |
NC_000067.6 |
1 |
161055418 |
- |
Dars2 |
NNNAGAACTGGAGTTTGAGG |
NGG |
2 |
0.0486 |
Tier II |
| 5 |
NC_000072.6 |
6 |
101309843 |
+ |
Pdzrn3 |
NNNAGAACTCGAGTTTGAGG |
NTG |
2 |
0.0049 |
Tier II |
Other clones with same target sequence:
(none)
