Construct: sgRNA BRDN0001145878
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTATGGCGCACTGTTTCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K6 (5608)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76039
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
69519428 |
+ |
MAP2K6 |
NNNTGGCGCACTGTTTCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
1394303 |
- |
CNTN6 |
NNNTGGAGCCCTGTTTCGGG |
NGG |
2 |
0.25 |
Tier II |
| 3 |
NC_000022.11 |
22 |
22391091 |
- |
IGL |
NNNTGCCGCAATGTTTCGGG |
NGG |
2 |
0.5114 |
Tier III |
| 4 |
NC_000003.12 |
3 |
1394303 |
- |
CRB3P1 |
NNNTGGAGCCCTGTTTCGGG |
NGG |
2 |
0.25 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
18618881 |
+ |
Arfgef3 |
NNNTGGCACACTGTTTGGGG |
NGG |
2 |
0.0588 |
Tier II |
| 2 |
NC_000074.6 |
8 |
28694224 |
+ |
Unc5d |
NNNTGGGGCACTGTTTGGGG |
NGG |
2 |
0.0277 |
Tier II |
| 3 |
NC_000083.6 |
17 |
25650558 |
- |
Lmf1 |
NNNTGGAGCACTGTTTCGGG |
NGT |
2 |
0.0121 |
Tier II |
| 4 |
NC_000071.6 |
5 |
104086662 |
- |
Sparcl1 |
NNNTGGCTCACTGTTTCGGG |
NGT |
2 |
0.0101 |
Tier II |
Other clones with same target sequence:
(none)
