Construct: sgRNA BRDN0001145887
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCTAGACAGCACCGTAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- JAK1 (3716)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76392
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
64844774 |
+ |
JAK1 |
NNNTAGACAGCACCGTAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
12482975 |
- |
CAMK1D |
NNNTAGCTAGCACCGTAATG |
NGG |
2 |
0.3828 |
Tier II |
| 3 |
NC_000010.11 |
10 |
55029641 |
+ |
PCDH15 |
NNNTAGAAAGCACCCTAATG |
NGG |
2 |
0.1773 |
Tier II |
| 4 |
NC_000003.12 |
3 |
188461781 |
+ |
LPP |
NNNTAGGCAGCACCTTAATG |
NGG |
2 |
0.1008 |
Tier II |
| 5 |
NC_000001.11 |
1 |
220260997 |
+ |
RAB3GAP2 |
NNNTAGACAGCACAGTACTG |
NGG |
2 |
0.0667 |
Tier II |
| 6 |
NC_000007.14 |
7 |
24853249 |
+ |
OSBPL3 |
NNNTAGACAGCACCTTAATC |
NGG |
2 |
0.0612 |
Tier II |
| 7 |
NC_000013.11 |
13 |
92114849 |
+ |
GPC5 |
NNNTAGACAGCAGGGTAATG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
162937075 |
+ |
Fmo6 |
NNNCAGACAGCACCGTATTG |
NGG |
2 |
0.3235 |
Tier I |
| 2 |
NC_000071.6 |
5 |
129753258 |
+ |
Nipsnap2 |
NNNTAGACAGGACAGTAATG |
NGG |
2 |
0.0875 |
Tier I |
| 3 |
NC_000071.6 |
5 |
51867043 |
- |
Ppargc1a |
NNNTAGACAGCACCGTCTTG |
NGG |
2 |
0.0882 |
Tier II |
| 4 |
NC_000075.6 |
9 |
16491640 |
+ |
Fat3 |
NNNTAGACAGCACCTTCATG |
NGG |
2 |
0.0252 |
Tier II |
| 5 |
NC_000067.6 |
1 |
162937075 |
+ |
Gm32279 |
NNNCAGACAGCACCGTATTG |
NGG |
2 |
0.3235 |
Tier III |
Other clones with same target sequence:
(none)
