Construct: sgRNA BRDN0001145897

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAATAGCAGCAATTACACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAOK1 (57551)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76807

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 29478294 + TAOK1 NNNATAGCAGCAATTACACA NGG 0 1.0 Tier I
2 NC_000013.11 13 46044960 - ZC3H13 NNNATAGCAGCAATTATAGA NGG 2 0.0583 Tier I
3 NC_000018.10 18 13680470 + FAM210A NNNACAGCATCAATTACACA NGG 2 0.8125 Tier II
4 NC_000017.11 17 68549831 - PRKAR1A NNNATAACAGCAAATACACA NGG 2 0.619 Tier II
5 NC_000017.11 17 68549831 - FAM20A NNNATAACAGCAAATACACA NGG 2 0.619 Tier II
6 NC_000007.14 7 102187427 - CUX1 NNNATAACAGCAATTACTCA NGG 2 0.5 Tier II
7 NC_000019.10 19 11610624 - ZNF627 NNNTTAGCAGCAGTTACACA NGG 2 0.415 Tier II
8 NC_000007.14 7 147877342 - CNTNAP2 NNNATAGCAGCTATTACACA NGG 1 0.3333 Tier II
9 NC_000016.10 16 22019043 - MOSMO NNNATAACAGCACTTACACA NGG 2 0.2105 Tier II
10 NC_000011.10 11 12215371 - MICAL2 NNNATATCAGCATTTACACA NGG 2 0.1714 Tier II
11 NC_000012.12 12 132742361 + ANKLE2 NNNATAACAGCAATTACAGA NGG 2 0.125 Tier II
12 NC_000017.11 17 17830121 - SREBF1 NNNTTAGCAGCAATTACCCA NGG 2 0.1212 Tier II
13 NC_000015.10 15 31815247 + OTUD7A NNNATAGCAGCAATTACATA NAG 2 0.1197 Tier II
14 NC_000016.10 16 23148070 + USP31 NNNATAGCAGCAAATACACA NGA 2 0.043 Tier II
15 NC_000008.11 8 135596563 - KHDRBS3 NNNATATCAGCAATTACACA NGA 2 0.0397 Tier II
16 NC_000008.11 8 91028066 + PIP4P2 NNNATAGCAGCAATTAAACA NTG 2 0.0182 Tier II
17 NC_000005.10 5 90913287 + ADGRV1 NNNATAGCAGCAATTACACT NGC 2 0.0133 Tier II
18 NC_000001.11 1 110618481 - KCNA2 NNNAGAGCAGCAATTCCACA NGG 2 0.0 Tier II
19 NC_000011.10 11 9589141 - WEE1 NNNATAGCAGCAAATTCACA NGG 2 0.0 Tier II
20 NC_000003.12 3 143528697 + SLC9A9 NNNATAGCAGCAATTTCATA NGG 2 0.0 Tier II
21 NC_000004.12 4 118949350 - SYNPO2 NNNATAGCAGCAATTTCATA NGG 2 0.0 Tier II
22 NC_000018.10 18 36513249 - FHOD3 NNNATAGCAGCAATTTCATA NGG 2 0.0 Tier II
23 NC_000009.12 9 107373099 + LOC107987111 NNNATAACAACAATTACACA NGG 2 0.9333 Tier III
24 NC_000001.11 1 212262501 + LINC02608 NNNATAATAGCAATTACACA NGG 2 0.875 Tier III
25 NC_000024.10 Y 18461180 - OFD1P4Y NNNATAGCATAAATTACACA NGG 2 0.6094 Tier III
26 NC_000024.10 Y 18859218 + OFD1P7Y NNNATAGCATAAATTACACA NGG 2 0.6094 Tier III
27 NC_000001.11 1 205968408 - SLC26A9-AS1 NNNATTCCAGCAATTACACA NGG 2 0.4911 Tier III
28 NC_000006.12 6 170678959 + LOC101929692 NNNATAGCTGAAATTACACA NGG 2 0.45 Tier III
29 NC_000019.10 19 20135558 + LOC105372310 NNNTTAGCAGCAATTACAAA NGG 2 0.2727 Tier III
30 NC_000011.10 11 12215371 - LOC124902634 NNNATATCAGCATTTACACA NGG 2 0.1714 Tier III
31 NC_000002.12 2 103901123 + LINC01965 NNNATAGCTGCAATTACACA NGA 2 0.0417 Tier III
32 NC_000005.10 5 97534321 - LINC01340 NNNATAGCAGCAATAACACA NGA 2 0.0402 Tier III
33 NC_000022.11 22 22196278 + IGL NNNATAGCAGCAATCACACA NTG 2 0.0106 Tier III
34 NC_000022.11 22 22196278 + IGLV6-57 NNNATAGCAGCAATCACACA NTG 2 0.0106 Tier III
35 NC_000014.9 14 48869627 - LOC105378178 NNNACAGCAGCAATTTCACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 77575627 - Taok1 NNNATAGCAGCAATTACACA NGG 0 1.0 Tier I
2 NC_000080.6 14 75292283 + Zc3h13 NNNACAGCAGCAATTACAGA NGG 2 0.125 Tier I
3 NC_000079.6 13 30864923 + Exoc2 NNNATAGCAGCAATTACTCA NGA 2 0.0347 Tier I
4 NC_000074.6 8 14598786 - Dlgap2 NNNATAGCATCAAATACACA NGG 2 0.503 Tier II
5 NC_000079.6 13 77194932 - 2210408I21Rik NNNATAGCAGCAATTACTCA NGG 1 0.5 Tier II
6 NC_000084.6 18 71947997 + Dcc NNNATATCAGCGATTACACA NGG 2 0.4127 Tier II
7 NC_000067.6 1 25514417 + Adgrb3 NNNATAGCAGAAATTATACA NGG 2 0.35 Tier II
8 NC_000075.6 9 100720283 + Stag1 NNNAGAGCAGCAATTAAACA NGG 2 0.2987 Tier II
9 NC_000078.6 12 37323407 - Agmo NNNTTAGCAGCAATTATACA NGG 2 0.297 Tier II
10 NC_000067.6 1 66204459 - Map2 NNNATAACAGCAATCACACA NGG 2 0.2727 Tier II
11 NC_000067.6 1 87346335 - Gigyf2 NNNATAACAGCAATTACACA NAG 2 0.2593 Tier II
12 NC_000068.7 2 15058685 - Arl5b NNNATACCAGCAATTACACA NAG 2 0.1782 Tier II
13 NC_000067.6 1 92155830 + Hdac4 NNNATAGCAGCAAATACCCA NGG 2 0.1179 Tier II
14 NC_000084.6 18 7898109 - Wac NNNATAGCAGAAATTACACA NGA 2 0.0521 Tier II
15 NC_000084.6 18 44547156 + Mcc NNNATAGCCGCAATTACACA NGT 2 0.0092 Tier II
16 NC_000068.7 2 140182781 + Ndufaf5 NNNAAAGCAGCAATTTCACA NGG 2 0.0 Tier II
17 NC_000073.6 7 110142663 - Wee1 NNNATAGCAGCAAATTCACA NGG 2 0.0 Tier II
18 NC_000085.6 19 38599002 + Plce1 NNNGTAGCAGCAATTTCACA NGG 2 0.0 Tier II
19 NC_000081.6 15 93722693 + Gm30339 NNNATAACAGCAATTACAGA NGG 2 0.125 Tier III
20 NC_000084.6 18 44547156 + Gm52347 NNNATAGCCGCAATTACACA NGT 2 0.0092 Tier III
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Other clones with same target sequence:

(none)