Construct: sgRNA BRDN0001145899
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAATCCTTATGAAACAGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K13 (9175)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76905
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
185450054 |
+ |
MAP3K13 |
NNNTCCTTATGAAACAGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
175622930 |
- |
TNR |
NNNTCCTTATAAAACAGACA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000018.10 |
18 |
35249180 |
+ |
ZNF397 |
NNNTCCTTATAAAACAGACA |
NGG |
2 |
0.9375 |
Tier II |
| 4 |
NC_000009.12 |
9 |
14347733 |
+ |
NFIB |
NNNTCCTTAGGAAACAGACA |
NGG |
2 |
0.4688 |
Tier II |
| 5 |
NC_000006.12 |
6 |
55829167 |
- |
BMP5 |
NNNTCCTTAGGAAACAAACG |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000018.10 |
18 |
68675933 |
+ |
TMX3 |
NNNTCCTTATGAAGCAGACA |
NGG |
2 |
0.4375 |
Tier II |
| 7 |
NC_000010.11 |
10 |
131102909 |
- |
TCERG1L |
NNNTCCATATCAAACAGACG |
NGG |
2 |
0.375 |
Tier II |
| 8 |
NC_000009.12 |
9 |
133972592 |
+ |
VAV2 |
NNNTCCTTAAGAAACAGAAG |
NGG |
2 |
0.3673 |
Tier II |
| 9 |
NC_000009.12 |
9 |
114488907 |
+ |
WHRN |
NNNTCCTTATAAAACATACG |
NGG |
2 |
0.25 |
Tier II |
| 10 |
NC_000020.11 |
20 |
15458001 |
- |
MACROD2 |
NNNTCCTTATGAATCAGATG |
NGG |
2 |
0.2462 |
Tier II |
| 11 |
NC_000019.10 |
19 |
35343488 |
+ |
CD22 |
NNNTCCTCATGACACAGACG |
NGG |
2 |
0.2105 |
Tier II |
| 12 |
NC_000003.12 |
3 |
129441628 |
+ |
IFT122 |
NNNTCCTTATGTTACAGACG |
NGG |
2 |
0.1 |
Tier II |
| 13 |
NC_000010.11 |
10 |
116198429 |
- |
GFRA1 |
NNNTCCTTATTAAACAGCCG |
NGG |
2 |
0.0733 |
Tier II |
| 14 |
NC_000005.10 |
5 |
114487937 |
+ |
KCNN2 |
NNNTCCTTATGAAACCTACG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000009.12 |
9 |
14347733 |
+ |
NFIB-AS1 |
NNNTCCTTAGGAAACAGACA |
NGG |
2 |
0.4688 |
Tier III |
| 16 |
NC_000014.9 |
14 |
97965119 |
- |
LINC01550 |
NNNTCCTTATGAATCAGATG |
NGG |
2 |
0.2462 |
Tier III |
| 17 |
NC_000009.12 |
9 |
134541633 |
- |
LOC100506532 |
NNNTCCTCATGACACAGACG |
NGG |
2 |
0.2105 |
Tier III |
| 18 |
NC_000012.12 |
12 |
126157182 |
+ |
LINC02359 |
NNNTCCTTATGAAACAGGCC |
NGG |
2 |
0.1714 |
Tier III |
| 19 |
NC_000012.12 |
12 |
126157182 |
+ |
LOC107984447 |
NNNTCCTTATGAAACAGGCC |
NGG |
2 |
0.1714 |
Tier III |
| 20 |
NC_000010.11 |
10 |
118503051 |
+ |
LOC105378507 |
NNNTCCTTATGAAATAGACA |
NGG |
2 |
0.0625 |
Tier III |
| 21 |
NC_000005.10 |
5 |
114487937 |
+ |
LOC101927078 |
NNNTCCTTATGAAACCTACG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
21905915 |
+ |
Map3k13 |
NNNTCCTTATGAAACAGACA |
NGG |
1 |
0.9375 |
Tier I |
| 2 |
NC_000073.6 |
7 |
16561511 |
- |
Arhgap35 |
NNNTCCATATGAAACAGATG |
NGG |
2 |
0.4038 |
Tier I |
| 3 |
NC_000074.6 |
8 |
8617492 |
+ |
Efnb2 |
NNNTCATTTTGAAACAGACG |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000077.6 |
11 |
66380611 |
+ |
Shisa6 |
NNNGCCTTAAGAAACAGACG |
NGG |
2 |
0.5357 |
Tier II |
| 5 |
NC_000078.6 |
12 |
83267344 |
+ |
Dpf3 |
NNNTTCTTATGAAACAGAAG |
NGG |
2 |
0.2727 |
Tier II |
| 6 |
NC_000073.6 |
7 |
45946735 |
- |
Ccdc114 |
NNNACCTTATGAAACAGATG |
NGG |
2 |
0.2198 |
Tier II |
| 7 |
NC_000068.7 |
2 |
156287410 |
- |
Phf20 |
NNNTCCTTATGAAACCGAAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000071.6 |
5 |
72260931 |
+ |
Atp10d |
NNNTCCTCAGGAAACAGACG |
NGG |
2 |
0.5 |
Tier III |
| 9 |
NC_000073.6 |
7 |
38425745 |
- |
Gm28453 |
NNNTCCTTCTGAAACAGAAG |
NGG |
2 |
0.2449 |
Tier III |
| 10 |
NC_000073.6 |
7 |
38843160 |
- |
1700010N08Rik |
NNNTCCTTCTGAAACAGAAG |
NGG |
2 |
0.2449 |
Tier III |
| 11 |
NC_000073.6 |
7 |
41310723 |
+ |
Topbp1-ps1 |
NNNTCCTTCTGAAACAGAAG |
NGG |
2 |
0.2449 |
Tier III |
| 12 |
NC_000084.6 |
18 |
65542242 |
- |
Gm41759 |
NNNTCCTTATGAAAGGGACG |
NGG |
2 |
0.0096 |
Tier III |
Other clones with same target sequence:
(none)
