Construct: sgRNA BRDN0001145903
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GACTTGGTCGATGATCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRA (5156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75492
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
54272428 |
+ |
PDGFRA |
NNNTTGGTCGATGATCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
64080336 |
+ |
LOC124902439 |
NNNTTGGTAGATGAGCACCA |
NGG |
2 |
0.0429 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
81273855 |
- |
Tjp3 |
NNNTGGGTAGATGATCACCA |
NGG |
2 |
0.5486 |
Tier I |
| 2 |
NC_000069.6 |
3 |
75923726 |
+ |
Golim4 |
NNNTTAGTCCATGATCACCA |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000076.6 |
10 |
50614081 |
- |
Ascc3 |
NNNTTAGTCCATGATCACCA |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000083.6 |
17 |
66876332 |
- |
Ptprm |
NNNTTAGTCCATGATCACCA |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000086.7 |
X |
141487481 |
+ |
Col4a5 |
NNNTTAGTCCATGATCACCA |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000084.6 |
18 |
21600159 |
- |
Klhl14 |
NNNTTGGTCCATGATCACCA |
NGG |
1 |
0.4 |
Tier II |
| 7 |
NC_000075.6 |
9 |
69490972 |
+ |
Anxa2 |
NNNTTGGTCTATGGTCACCA |
NGG |
2 |
0.3792 |
Tier II |
| 8 |
NC_000085.6 |
19 |
46965994 |
+ |
Nt5c2 |
NNNTTGGTCCATGATCACCG |
NGG |
2 |
0.3059 |
Tier II |
| 9 |
NC_000068.7 |
2 |
112662305 |
- |
Ryr3 |
NNNTTGGTCCATGATCATCA |
NGG |
2 |
0.2571 |
Tier II |
| 10 |
NC_000080.6 |
14 |
32867023 |
+ |
Vstm4 |
NNNTTGGTCCATGATCATCA |
NGG |
2 |
0.2571 |
Tier II |
| 11 |
NC_000070.6 |
4 |
74353125 |
+ |
Kdm4c |
NNNTTGGTCCATGACCACCA |
NGG |
2 |
0.1091 |
Tier II |
| 12 |
NC_000080.6 |
14 |
62860733 |
- |
Wdfy2 |
NNNTTGGTCCATGACCACCA |
NGG |
2 |
0.1091 |
Tier II |
| 13 |
NC_000081.6 |
15 |
67234913 |
- |
1700012I11Rik |
NNNTTGGTCGATGATCACCT |
NGG |
1 |
0.6 |
Tier III |
| 14 |
NC_000077.6 |
11 |
109624899 |
- |
Gm11685 |
NNNTTAGTCCATGATCACCA |
NGG |
2 |
0.4 |
Tier III |
| 15 |
NC_000081.6 |
15 |
26452026 |
+ |
Gm36790 |
NNNTTAGTCCATGATCACCA |
NGG |
2 |
0.4 |
Tier III |
| 16 |
NC_000084.6 |
18 |
21600159 |
- |
Gm31646 |
NNNTTGGTCCATGATCACCA |
NGG |
1 |
0.4 |
Tier III |
| 17 |
NC_000074.6 |
8 |
113385535 |
- |
Gm29955 |
NNNTTGCTCCATGATCACCA |
NGG |
2 |
0.275 |
Tier III |
| 18 |
NC_000078.6 |
12 |
27981264 |
+ |
Gm45941 |
NNNTTGGTCCATGATCATCA |
NGG |
2 |
0.2571 |
Tier III |
| 19 |
NC_000072.6 |
6 |
57466037 |
- |
4930533I22Rik |
NNNTTGGTCCATTATCACCA |
NGG |
2 |
0.12 |
Tier III |
| 20 |
NC_000084.6 |
18 |
45136694 |
+ |
Gm31706 |
NNNTTGGTTGATGATCTCCA |
NGG |
2 |
0.1167 |
Tier III |
| 21 |
NC_000077.6 |
11 |
32841114 |
- |
Gm35723 |
NNNTTGGTCGATGCCCACCA |
NGG |
2 |
0.0584 |
Tier III |
Other clones with same target sequence:
(none)
