Construct: sgRNA BRDN0001145904
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTCATACCTGAACAACCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROCK1 (6093)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77594
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
21049101 |
+ |
ROCK1 |
NNNCATACCTGAACAACCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
11249670 |
+ |
ROCK2 |
NNNCTTACCTGAACCACCCA |
NGG |
2 |
0.0992 |
Tier I |
| 3 |
NC_000010.11 |
10 |
97861140 |
- |
GOLGA7B |
NNNCATATCTGAATAACCCA |
NGG |
2 |
0.6417 |
Tier II |
| 4 |
NC_000004.12 |
4 |
167192862 |
- |
SPOCK3 |
NNNCATACCTGAACAACCAA |
NGG |
1 |
0.4286 |
Tier II |
| 5 |
NC_000012.12 |
12 |
27414554 |
+ |
BMAL2 |
NNNCATACCTGAACAACCAG |
NGG |
2 |
0.3277 |
Tier II |
| 6 |
NC_000019.10 |
19 |
12275842 |
- |
ZNF44 |
NNNCATTCCTGAACAACACA |
NGG |
2 |
0.2356 |
Tier II |
| 7 |
NC_000002.12 |
2 |
182408283 |
+ |
PDE1A |
NNNCATACCTGGACTACCCA |
NGG |
2 |
0.1444 |
Tier II |
| 8 |
NC_000015.10 |
15 |
94439937 |
- |
MCTP2 |
NNNCATACCTGAAAAGCCCA |
NGG |
2 |
0.0673 |
Tier II |
| 9 |
NC_000002.12 |
2 |
196583218 |
+ |
HECW2 |
NNNCATACCTGAACTACCCC |
NGG |
2 |
0.0455 |
Tier II |
| 10 |
NC_000003.12 |
3 |
87837232 |
+ |
HTR1F |
NNNCATAACTGAACAAGCCA |
NGG |
2 |
0.0382 |
Tier II |
| 11 |
NC_000001.11 |
1 |
47030316 |
- |
CYP4X1 |
NNNCATACCTGAACAAGACA |
NGG |
2 |
0.0317 |
Tier II |
| 12 |
NC_000018.10 |
18 |
45377050 |
+ |
SLC14A2 |
NNNCATGCCTGAACAACCCA |
NTG |
2 |
0.0275 |
Tier II |
| 13 |
NC_000002.12 |
2 |
178270017 |
- |
OSBPL6 |
NNNCATACCTGAACAAGCCA |
NTG |
2 |
0.0023 |
Tier II |
| 14 |
NC_000015.10 |
15 |
86431917 |
+ |
AGBL1 |
NNNCAGACCTGAACACCCCA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000010.11 |
10 |
86819790 |
- |
BMPR1A |
NNNCATAACTGAAGAACCCA |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000010.11 |
10 |
58545844 |
+ |
BICC1 |
NNNCATACCTGAACAACCCA |
NAC |
2 |
0.0 |
Tier II |
| 17 |
NC_000002.12 |
2 |
51299373 |
- |
NRXN1-DT |
NNNCAGAGCTGAACAACCCA |
NGG |
2 |
0.3673 |
Tier III |
| 18 |
NC_000012.12 |
12 |
27414554 |
+ |
BMAL2-AS1 |
NNNCATACCTGAACAACCAG |
NGG |
2 |
0.3277 |
Tier III |
| 19 |
NC_000016.10 |
16 |
72711744 |
- |
ZFHX3-AS1 |
NNNCATACATGAACAACCCA |
NAG |
2 |
0.2222 |
Tier III |
| 20 |
NC_000004.12 |
4 |
148623685 |
+ |
LOC105377483 |
NNNCATTCCTGAACAACCAA |
NGG |
2 |
0.1875 |
Tier III |
| 21 |
NC_000004.12 |
4 |
148623685 |
+ |
LOC107986195 |
NNNCATTCCTGAACAACCAA |
NGG |
2 |
0.1875 |
Tier III |
| 22 |
NC_000001.11 |
1 |
205557752 |
+ |
MFSD4A-AS1 |
NNNCATACCTGAACAACCAC |
NGG |
2 |
0.0974 |
Tier III |
| 23 |
NC_000018.10 |
18 |
45377050 |
+ |
SLC14A2-AS1 |
NNNCATGCCTGAACAACCCA |
NTG |
2 |
0.0275 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
10140185 |
+ |
Rock1 |
NNNGATACCTGAACAACCCA |
NGG |
1 |
0.5 |
Tier I |
| 2 |
NC_000078.6 |
12 |
16940532 |
- |
Rock2 |
NNNCTTACCTGAACTACCCA |
NGG |
2 |
0.0727 |
Tier I |
| 3 |
NC_000071.6 |
5 |
150173448 |
+ |
Fry |
NNNCACACATGAACAACCCA |
NGG |
2 |
0.7792 |
Tier II |
| 4 |
NC_000074.6 |
8 |
84165575 |
+ |
4930432K21Rik |
NNNCATACCTGAACAACACA |
NGG |
1 |
0.5385 |
Tier II |
| 5 |
NC_000079.6 |
13 |
95331837 |
- |
Zbed3 |
NNNTATACCTGAACAACTCA |
NGG |
2 |
0.5143 |
Tier II |
| 6 |
NC_000070.6 |
4 |
138065689 |
+ |
Eif4g3 |
NNNAATACCTGAACAACCAA |
NGG |
2 |
0.3609 |
Tier II |
| 7 |
NC_000080.6 |
14 |
12009813 |
+ |
Ptprg |
NNNCATAACTGAAAAACCCA |
NGG |
2 |
0.2275 |
Tier II |
| 8 |
NC_000079.6 |
13 |
10341612 |
- |
Chrm3 |
NNNCATGCCTGAACAACCCA |
NAG |
2 |
0.183 |
Tier II |
| 9 |
NC_000072.6 |
6 |
51263795 |
- |
Gm38821 |
NNNCACACCTGAACAGCCCA |
NGG |
2 |
0.1748 |
Tier II |
| 10 |
NC_000082.6 |
16 |
31886586 |
- |
Meltf |
NNNCATACCAGAACAACCCA |
NCG |
2 |
0.0918 |
Tier II |
| 11 |
NC_000085.6 |
19 |
24403612 |
- |
Pip5k1b |
NNNCATACCTGAACAAGCTA |
NGG |
2 |
0.0271 |
Tier II |
| 12 |
NC_000079.6 |
13 |
18332561 |
- |
Pou6f2 |
NNNCATACCTGAACACCCCA |
NTG |
2 |
0.0 |
Tier II |
| 13 |
NC_000068.7 |
2 |
147266715 |
+ |
Nkx2-2os |
NNNCATACCTGAACAATCGA |
NGG |
2 |
0.0583 |
Tier III |
Other clones with same target sequence:
(none)
