Construct: sgRNA BRDN0001145911

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTGGTATATGGACGCTGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1 (6872)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77870

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 71381824 + TAF1 NNNGGTATATGGACGCTGGG NGG 0 1.0 Tier I
2 NC_000009.12 9 32634085 - TAF1L NNNGGTGTATGGACGCTGGG NGG 1 0.7059 Tier I
3 NC_000015.10 15 41872610 - SPTBN5 NNNGGTAGATGGATGCTGGG NGG 2 0.5378 Tier II
4 NC_000013.11 13 98860731 + DOCK9 NNNGGGATATGGATGCTGGG NGG 2 0.419 Tier II
5 NC_000002.12 2 3207167 - EIPR1 NNNGGTAGATGGACGGTGGG NGG 2 0.1128 Tier II
6 NC_000012.12 12 12455301 - BORCS5 NNNGGAATATGGAGGCTGGG NGG 2 0.0 Tier II
7 NC_000010.11 10 98908406 + HPSE2 NNNGGTATATGGAGGCTGGG NGC 2 0.0 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 101547040 + Taf1 NNNGGTATATGGACGTTGGG NGG 1 0.3077 Tier I
2 NC_000070.6 4 115939223 + Dmbx1 NNNGGAATAGGGACGCTGGG NGG 2 0.4333 Tier II
3 NC_000077.6 11 59493676 - Zkscan17 NNNGGTATATGGAAGGTGGG NGG 2 0.0538 Tier II
4 NC_000072.6 6 82029638 + Gm32378 NNNTGTATATGGACGCTGGG NGC 2 0.0081 Tier III
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Other clones with same target sequence:

(none)