Construct: sgRNA BRDN0001145911
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGTATATGGACGCTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAF1 (6872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77870
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
71381824 |
+ |
TAF1 |
NNNGGTATATGGACGCTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
32634085 |
- |
TAF1L |
NNNGGTGTATGGACGCTGGG |
NGG |
1 |
0.7059 |
Tier I |
| 3 |
NC_000015.10 |
15 |
41872610 |
- |
SPTBN5 |
NNNGGTAGATGGATGCTGGG |
NGG |
2 |
0.5378 |
Tier II |
| 4 |
NC_000013.11 |
13 |
98860731 |
+ |
DOCK9 |
NNNGGGATATGGATGCTGGG |
NGG |
2 |
0.419 |
Tier II |
| 5 |
NC_000002.12 |
2 |
3207167 |
- |
EIPR1 |
NNNGGTAGATGGACGGTGGG |
NGG |
2 |
0.1128 |
Tier II |
| 6 |
NC_000012.12 |
12 |
12455301 |
- |
BORCS5 |
NNNGGAATATGGAGGCTGGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000010.11 |
10 |
98908406 |
+ |
HPSE2 |
NNNGGTATATGGAGGCTGGG |
NGC |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
101547040 |
+ |
Taf1 |
NNNGGTATATGGACGTTGGG |
NGG |
1 |
0.3077 |
Tier I |
| 2 |
NC_000070.6 |
4 |
115939223 |
+ |
Dmbx1 |
NNNGGAATAGGGACGCTGGG |
NGG |
2 |
0.4333 |
Tier II |
| 3 |
NC_000077.6 |
11 |
59493676 |
- |
Zkscan17 |
NNNGGTATATGGAAGGTGGG |
NGG |
2 |
0.0538 |
Tier II |
| 4 |
NC_000072.6 |
6 |
82029638 |
+ |
Gm32378 |
NNNTGTATATGGACGCTGGG |
NGC |
2 |
0.0081 |
Tier III |
Other clones with same target sequence:
(none)
