Construct: sgRNA BRDN0001145913
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCCCCCGAATCATTCCTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FYN (2534)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77245
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
111696385 |
- |
FYN |
NNNCCCCGAATCATTCCTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
53550646 |
- |
WDR72 |
NNNTCCCTAATCATTCCTTG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000003.12 |
3 |
42528804 |
+ |
VIPR1 |
NNNCCCCCACTCATTCCTTG |
NGG |
2 |
0.2051 |
Tier II |
| 4 |
NC_000020.11 |
20 |
37240107 |
+ |
RPN2 |
NNNCCCCAAATCATTGCTTG |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000004.12 |
4 |
186228897 |
- |
KLKB1 |
NNNCCCAGAATCATGCCTTG |
NGG |
2 |
0.0375 |
Tier II |
| 6 |
NC_000006.12 |
6 |
31881486 |
- |
EHMT2 |
NNNCCCCGAATCATGCCTTG |
NGT |
2 |
0.0008 |
Tier II |
| 7 |
NC_000015.10 |
15 |
53550646 |
- |
LOC105370826 |
NNNTCCCTAATCATTCCTTG |
NGG |
2 |
0.5 |
Tier III |
| 8 |
NC_000009.12 |
9 |
76598825 |
+ |
LOC105376095 |
NNNCCCCGAATCAATCCTAG |
NGG |
2 |
0.1769 |
Tier III |
| 9 |
NC_000006.12 |
6 |
31881486 |
- |
EHMT2-AS1 |
NNNCCCCGAATCATGCCTTG |
NGT |
2 |
0.0008 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
87477642 |
+ |
Ngef |
NNNCCCGGAAACATTCCTTG |
NGG |
2 |
0.3529 |
Tier I |
| 2 |
NC_000077.6 |
11 |
76447546 |
- |
Abr |
NNNCCCAGAGTCATTCCTTG |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000072.6 |
6 |
97975301 |
+ |
Mitf |
NNNCCCCGAATCATTGCATG |
NGG |
2 |
0.1026 |
Tier II |
| 4 |
NC_000067.6 |
1 |
34011711 |
+ |
Dst |
NNNCCCCGAAGCCTTCCTTG |
NGG |
2 |
0.0842 |
Tier II |
| 5 |
NC_000075.6 |
9 |
87179046 |
- |
Mrap2 |
NNNCCCCGAATCAGTCCATG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000068.7 |
2 |
166565015 |
+ |
Gm29679 |
NNNCCCCGAATCATTTCTGG |
NGG |
2 |
0.0769 |
Tier III |
Other clones with same target sequence:
(none)
