Construct: sgRNA BRDN0001145914
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCCAGTTACCTATAGCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4KA (5297)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76818
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
20798590 |
+ |
PI4KA |
NNNCCAGTTACCTATAGCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
84452086 |
- |
ATP2C2 |
NNNCCAGTTACCTATGGCCA |
NGG |
2 |
0.1803 |
Tier I |
| 3 |
NC_000005.10 |
5 |
73583161 |
- |
UTP15 |
NNNCCAGTTAACTATAGCTG |
NGG |
2 |
0.3462 |
Tier II |
| 4 |
NC_000001.11 |
1 |
36370049 |
- |
STK40 |
NNNCCAGTTACCTACAGCTG |
NGG |
2 |
0.1259 |
Tier II |
| 5 |
NC_000004.12 |
4 |
33898742 |
- |
LOC101928622 |
NNNCCAGTTACCTATAGAAG |
NGG |
2 |
0.2308 |
Tier III |
| 6 |
NC_000004.12 |
4 |
33898742 |
- |
LOC105374392 |
NNNCCAGTTACCTATAGAAG |
NGG |
2 |
0.2308 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
79556829 |
- |
Nf1 |
NNNCCACTTACCTATAGCTG |
NGG |
2 |
0.3173 |
Tier I |
| 2 |
NC_000084.6 |
18 |
69663418 |
+ |
Tcf4 |
NNNCCAGTGACCTATAGCCC |
NGG |
2 |
0.2653 |
Tier II |
| 3 |
NC_000071.6 |
5 |
100390994 |
+ |
Sec31a |
NNNCCAGTTCCCTATAGCTG |
NGG |
2 |
0.1538 |
Tier II |
| 4 |
NC_000084.6 |
18 |
64940008 |
+ |
Nedd4l |
NNNCCAGTTGCCTACAGCCG |
NGG |
2 |
0.1515 |
Tier II |
| 5 |
NC_000079.6 |
13 |
89290790 |
+ |
Edil3 |
NNNCCAGTTACCTATAGGCT |
NGG |
2 |
0.0933 |
Tier II |
Other clones with same target sequence:
(none)
