Construct: sgRNA BRDN0001145915

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTAGCGTGGGTAAATACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3CB (5291)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76192

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 138712280 - PIK3CB NNNAGCGTGGGTAAATACGA NGG 0 1.0 Tier I
2 NC_000009.12 9 74555846 + RORB NNNAGCGTGGGAAAATAAGA NGG 2 0.4308 Tier II
3 NC_000012.12 12 103834536 + NT5DC3 NNNAGGGTAGGTAAATACGA NGG 2 0.3214 Tier II
4 NC_000005.10 5 77046326 - AGGF1 NNNAGGGTGGGTAGATACGA NGG 2 0.2333 Tier II
5 NC_000010.11 10 31139717 - LOC105376481 NNNAGCGTGGGGAAATACGT NGG 2 0.3 Tier III
6 NC_000010.11 10 31139717 - LOC105376483 NNNAGCGTGGGGAAATACGT NGG 2 0.3 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 19058316 - Rorb NNNAGCGTGGGAAAATAAGA NGG 2 0.4308 Tier II
2 NC_000084.6 18 89099867 - Rttn NNNAGCGTGGGAAAATGCGA NGG 2 0.1412 Tier II
Download CSV

Other clones with same target sequence:

(none)