Construct: sgRNA BRDN0001145920
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGGTCATACCGTATTGCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK2 (4751)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76216
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
211673624 |
- |
NEK2 |
NNNGTCATACCGTATTGCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
180043117 |
- |
CEP350 |
NNNGTCATACAGTATTGCAA |
NGG |
2 |
0.4219 |
Tier I |
| 3 |
NC_000008.11 |
8 |
117012477 |
- |
SLC30A8 |
NNNGTCATACTGTATTGTAT |
NGG |
2 |
0.1978 |
Tier II |
| 4 |
NC_000012.12 |
12 |
1113044 |
- |
ERC1 |
NNNGTCATACTGTATTGCTT |
NGG |
2 |
0.1657 |
Tier II |
| 5 |
NC_000011.10 |
11 |
87301294 |
+ |
TMEM135 |
NNNGTCATACTGTATTGCCT |
NGG |
2 |
0.0637 |
Tier II |
| 6 |
NC_000012.12 |
12 |
80232244 |
- |
OTOGL |
NNNGTCATACAGTATTGCAT |
NGA |
2 |
0.0521 |
Tier II |
| 7 |
NC_000012.12 |
12 |
28128095 |
+ |
LOC105369710 |
NNNGTCACACTGTATTGCAT |
NGG |
2 |
0.3077 |
Tier III |
| 8 |
NC_000012.12 |
12 |
28128095 |
+ |
LOC107984462 |
NNNGTCACACTGTATTGCAT |
NGG |
2 |
0.3077 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
74645296 |
+ |
Myo5b |
NNNGTCAGACGGTATTGCAT |
NGG |
2 |
0.1833 |
Tier II |
| 2 |
NC_000079.6 |
13 |
49852881 |
- |
Gm272 |
NNNGTCATACAGTCTTGCAT |
NGG |
2 |
0.1607 |
Tier II |
Other clones with same target sequence:
(none)
