Construct: sgRNA BRDN0001145923
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATTGGCGAGTAAAGCACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRPPRC (10128)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77775
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
43973818 |
+ |
LRPPRC |
NNNTGGCGAGTAAAGCACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
31232169 |
- |
B3GLCT |
NNNTGGCAAATAAAGCACAA |
NGG |
2 |
0.9333 |
Tier II |
| 3 |
NC_000006.12 |
6 |
100658040 |
+ |
ASCC3 |
NNNTGCTGAGTAAAGCACAA |
NGG |
2 |
0.554 |
Tier II |
| 4 |
NC_000002.12 |
2 |
85193209 |
+ |
TCF7L1 |
NNNTGGCGAGTAGAGCACAG |
NGG |
2 |
0.4987 |
Tier II |
| 5 |
NC_000002.12 |
2 |
229699012 |
+ |
DNER |
NNNTGGAGAGTAAAGCATAA |
NGG |
2 |
0.4821 |
Tier II |
| 6 |
NC_000023.11 |
X |
129782021 |
+ |
SASH3 |
NNNTGGAGAGGAAAGCACAA |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000014.9 |
14 |
78446949 |
- |
NRXN3 |
NNNTGGCGAGTAAAGCTCAG |
NGG |
2 |
0.102 |
Tier II |
| 8 |
NC_000004.12 |
4 |
42311949 |
- |
LOC105374428 |
NNNTGGAGAGTAAGGCACAA |
NGG |
2 |
0.35 |
Tier III |
| 9 |
NC_000023.11 |
X |
129782021 |
+ |
LOC124905215 |
NNNTGGAGAGGAAAGCACAA |
NGG |
2 |
0.3 |
Tier III |
| 10 |
NC_000010.11 |
10 |
61476519 |
- |
TMEM26-AS1 |
NNNTGGGTAGTAAAGCACAA |
NGG |
2 |
0.2941 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
174226341 |
- |
Spta1 |
NNNTGGAAAGTAAAGCACAA |
NGG |
2 |
0.75 |
Tier II |
| 2 |
NC_000068.7 |
2 |
181764751 |
+ |
Myt1 |
NNNTGGCGAGTAGAGAACAA |
NGG |
2 |
0.6522 |
Tier II |
| 3 |
NC_000073.6 |
7 |
115669851 |
- |
Sox6 |
NNNTGGCTAATAAAGCACAA |
NGG |
2 |
0.5833 |
Tier II |
| 4 |
NC_000077.6 |
11 |
35851674 |
- |
Wwc1 |
NNNTGGGGAGTAAAGCACAG |
NGG |
2 |
0.3599 |
Tier II |
| 5 |
NC_000073.6 |
7 |
139473512 |
- |
Inpp5a |
NNNAGGGGAGTAAAGCACAA |
NGG |
2 |
0.2241 |
Tier II |
| 6 |
NC_000071.6 |
5 |
52074191 |
+ |
Ppargc1a |
NNNTGGCGAGTAAATCACAA |
NAG |
2 |
0.037 |
Tier II |
| 7 |
NC_000068.7 |
2 |
11664547 |
+ |
Il2ra |
NNNTGGCGAGAAAAGCACAA |
NTG |
2 |
0.0292 |
Tier II |
| 8 |
NC_000081.6 |
15 |
80925449 |
+ |
Tnrc6b |
NNNTGGCGAGTAAATCACAA |
NGT |
2 |
0.0023 |
Tier II |
| 9 |
NC_000076.6 |
10 |
121521558 |
+ |
Gm35696 |
NNNTGGCGAGCAAAGAACAA |
NGG |
2 |
0.6667 |
Tier III |
| 10 |
NC_000068.7 |
2 |
136623914 |
- |
Gm32659 |
NNNTGGGGAGTAAAGCACAA |
NGG |
1 |
0.4706 |
Tier III |
| 11 |
NC_000071.6 |
5 |
99427842 |
+ |
A930011G23Rik |
NNNTGCCGAGTACAGCACAA |
NGG |
2 |
0.1435 |
Tier III |
Other clones with same target sequence:
(none)
