Construct: sgRNA BRDN0001145930

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAAGCATATTATGTCGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK1 (6446)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77457

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 134173137 - SGK1 NNNAGCATATTATGTCGGAG NGG 0 1.0 Tier I
2 NC_000016.10 16 14534550 - PARN NNNAGCATATTATGTAGGGG NGG 2 0.375 Tier II
3 NC_000014.9 14 51728428 - FRMD6 NNNAGCATTTTATGTGGGAG NGG 2 0.0923 Tier II
4 NC_000023.11 X 12359265 + FRMPD4 NNNAGCATATTATGTGGGGG NGG 2 0.0577 Tier II
5 NC_000009.12 9 125349991 - GAPVD1 NNNAGCATATTATGGCAGAG NGG 2 0.0467 Tier II
6 NC_000012.12 12 70813019 - PTPRR NNNAGCAAATTATGTCGGAG NGT 2 0.0129 Tier II
7 NC_000016.10 16 14534550 - LOC107984865 NNNAGCATATTATGTAGGGG NGG 2 0.375 Tier III
8 NC_000011.10 11 131300701 - LOC124902794 NNNAGCATATTATGTTGGAA NGG 2 0.2885 Tier III
9 NC_000024.10 Y 18487348 - USP9YP14 NNNAGCATATTCTGTTGGAG NGG 2 0.081 Tier III
10 NC_000024.10 Y 18487348 - LOC124905304 NNNAGCATATTCTGTTGGAG NGG 2 0.081 Tier III
11 NC_000024.10 Y 18833055 + USP9YP28 NNNAGCATATTCTGTTGGAG NGG 2 0.081 Tier III
12 NC_000024.10 Y 18833055 + LOC124905305 NNNAGCATATTCTGTTGGAG NGG 2 0.081 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 21996241 + Sgk1 NNNAGCATATTATGTCAGAG NGG 1 0.9333 Tier I
2 NC_000067.6 1 14295209 + Eya1 NNNACCATATTATGTAGGAG NGG 2 0.7857 Tier II
3 NC_000081.6 15 50853180 - Trps1 NNNAGCATTTTATGTCAGAG NGG 2 0.56 Tier II
Download CSV

Other clones with same target sequence:

(none)