Construct: sgRNA BRDN0001145930
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAAGCATATTATGTCGGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SGK1 (6446)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77457
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
134173137 |
- |
SGK1 |
NNNAGCATATTATGTCGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
14534550 |
- |
PARN |
NNNAGCATATTATGTAGGGG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000014.9 |
14 |
51728428 |
- |
FRMD6 |
NNNAGCATTTTATGTGGGAG |
NGG |
2 |
0.0923 |
Tier II |
| 4 |
NC_000023.11 |
X |
12359265 |
+ |
FRMPD4 |
NNNAGCATATTATGTGGGGG |
NGG |
2 |
0.0577 |
Tier II |
| 5 |
NC_000009.12 |
9 |
125349991 |
- |
GAPVD1 |
NNNAGCATATTATGGCAGAG |
NGG |
2 |
0.0467 |
Tier II |
| 6 |
NC_000012.12 |
12 |
70813019 |
- |
PTPRR |
NNNAGCAAATTATGTCGGAG |
NGT |
2 |
0.0129 |
Tier II |
| 7 |
NC_000016.10 |
16 |
14534550 |
- |
LOC107984865 |
NNNAGCATATTATGTAGGGG |
NGG |
2 |
0.375 |
Tier III |
| 8 |
NC_000011.10 |
11 |
131300701 |
- |
LOC124902794 |
NNNAGCATATTATGTTGGAA |
NGG |
2 |
0.2885 |
Tier III |
| 9 |
NC_000024.10 |
Y |
18487348 |
- |
USP9YP14 |
NNNAGCATATTCTGTTGGAG |
NGG |
2 |
0.081 |
Tier III |
| 10 |
NC_000024.10 |
Y |
18487348 |
- |
LOC124905304 |
NNNAGCATATTCTGTTGGAG |
NGG |
2 |
0.081 |
Tier III |
| 11 |
NC_000024.10 |
Y |
18833055 |
+ |
USP9YP28 |
NNNAGCATATTCTGTTGGAG |
NGG |
2 |
0.081 |
Tier III |
| 12 |
NC_000024.10 |
Y |
18833055 |
+ |
LOC124905305 |
NNNAGCATATTCTGTTGGAG |
NGG |
2 |
0.081 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
21996241 |
+ |
Sgk1 |
NNNAGCATATTATGTCAGAG |
NGG |
1 |
0.9333 |
Tier I |
| 2 |
NC_000067.6 |
1 |
14295209 |
+ |
Eya1 |
NNNACCATATTATGTAGGAG |
NGG |
2 |
0.7857 |
Tier II |
| 3 |
NC_000081.6 |
15 |
50853180 |
- |
Trps1 |
NNNAGCATTTTATGTCAGAG |
NGG |
2 |
0.56 |
Tier II |
Other clones with same target sequence:
(none)
