Construct: sgRNA BRDN0001145932
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCAGAGGATTAGTGCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TJP2 (9414)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77829
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
69234470 |
+ |
TJP2 |
NNNCAGAGGATTAGTGCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
43718433 |
+ |
MAOA |
NNNCAGAGGATTAGTGTTGG |
NGG |
2 |
0.3111 |
Tier II |
| 3 |
NC_000011.10 |
11 |
64720468 |
+ |
NRXN2 |
NNNCTGAGGATTAATGCGGG |
NGG |
2 |
0.2727 |
Tier II |
| 4 |
NC_000013.11 |
13 |
51796828 |
- |
DHRS12 |
NNNCAGAGGCTTAGTGCTGG |
NGG |
2 |
0.2222 |
Tier II |
| 5 |
NC_000010.11 |
10 |
13971977 |
- |
FRMD4A |
NNNCTGAGGATTAGTGAGGG |
NGG |
2 |
0.1697 |
Tier II |
| 6 |
NC_000009.12 |
9 |
132057199 |
- |
MED27 |
NNNCAGAGGATTACCGCGGG |
NGG |
2 |
0.1169 |
Tier II |
| 7 |
NC_000016.10 |
16 |
50308268 |
+ |
ADCY7 |
NNNCAGAGGATTGGTGGGGG |
NGG |
2 |
0.0384 |
Tier II |
| 8 |
NC_000021.9 |
21 |
45556013 |
- |
SLC19A1 |
NNNCAGAGGATTAGGGCGGG |
NGT |
2 |
0.0008 |
Tier II |
| 9 |
NC_000017.11 |
17 |
76124401 |
- |
LOC101928447 |
NNNCAGAGGACAAGTGCGGG |
NGG |
2 |
0.5333 |
Tier III |
| 10 |
NC_000016.10 |
16 |
11316611 |
- |
LOC105371082 |
NNNCAGAGGGTTGGTGCGGG |
NGG |
2 |
0.3623 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
40306787 |
+ |
Gramd1b |
NNNCAGAGGATTAGTGAGGG |
NGA |
2 |
0.0324 |
Tier I |
| 2 |
NC_000081.6 |
15 |
68230247 |
- |
Zfat |
NNNCAGAGGACTGGTGCGGG |
NGG |
2 |
0.4348 |
Tier II |
| 3 |
NC_000082.6 |
16 |
35542018 |
+ |
Sema5b |
NNNCAGAGGAAGAGTGCGGG |
NGG |
2 |
0.375 |
Tier II |
| 4 |
NC_000077.6 |
11 |
75177548 |
- |
Ovca2 |
NNNCAGAGGATAAGTGCGGG |
NAG |
2 |
0.2074 |
Tier II |
| 5 |
NC_000081.6 |
15 |
25895375 |
+ |
Retreg1 |
NNNCAGCGGATTTGTGCGGG |
NGG |
2 |
0.1313 |
Tier II |
| 6 |
NC_000079.6 |
13 |
38410237 |
- |
Bmp6 |
NNNCAGAGGATTAGCGGGGG |
NGG |
2 |
0.016 |
Tier II |
Other clones with same target sequence:
(none)
