Construct: sgRNA BRDN0001145934
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGGAACTTCGCACCTGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K9 (4293)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77310
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
70742579 |
- |
MAP3K9 |
NNNGGAACTTCGCACCTGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
2853473 |
- |
PRSS22 |
NNNGGGACTTCTCACCTGGG |
NGG |
2 |
0.2747 |
Tier II |
| 3 |
NC_000007.14 |
7 |
7675928 |
- |
RPA3 |
NNNGGAGCTTTGCACCTGGG |
NGG |
2 |
0.2172 |
Tier II |
| 4 |
NC_000007.14 |
7 |
7675928 |
- |
UMAD1 |
NNNGGAGCTTTGCACCTGGG |
NGG |
2 |
0.2172 |
Tier II |
| 5 |
NC_000005.10 |
5 |
31680506 |
+ |
PDZD2 |
NNNGGAACTTCGCACTTGGG |
NAG |
2 |
0.0798 |
Tier II |
| 6 |
NC_000020.11 |
20 |
43788813 |
- |
LOC101927200 |
NNNGAAACTTCGAACCTGGG |
NGG |
2 |
0.3333 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
3782971 |
- |
Lyn |
NNNGGAACTTCGCACCTGAG |
NAG |
2 |
0.1852 |
Tier I |
| 2 |
NC_000069.6 |
3 |
51661926 |
- |
Mgst2 |
NNNGGAACTTCGCTCCTGGG |
NAG |
2 |
0.1383 |
Tier II |
| 3 |
NC_000073.6 |
7 |
110629241 |
+ |
Adm |
NNNAGAACTTCGCACCGGGG |
NGG |
2 |
0.1059 |
Tier II |
| 4 |
NC_000075.6 |
9 |
104758178 |
- |
Cpne4 |
NNNGGAACTTCACATCTGGG |
NGG |
2 |
0.0622 |
Tier II |
| 5 |
NC_000073.6 |
7 |
110629241 |
+ |
Gm28863 |
NNNAGAACTTCGCACCGGGG |
NGG |
2 |
0.1059 |
Tier III |
Other clones with same target sequence:
(none)
