Construct: sgRNA BRDN0001145935

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAGGCCCATATGAACAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP23 (91419)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77435

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 57951804 + ATP23 NNNGGCCCATATGAACAGAG NGG 0 1.0 Tier I
2 NC_000008.11 8 22920320 + PEBP4 NNNGGCCCCTATGAAGAGAG NGG 2 0.0879 Tier I
3 NC_000022.11 22 39099876 + APOBEC3H NNNGGCCCAAATGAACAGAA NGG 2 0.8036 Tier II
4 NC_000011.10 11 72016559 + NUMA1 NNNGACCCATGTGAACAGAG NGG 2 0.5633 Tier II
5 NC_000014.9 14 32769600 - AKAP6 NNNGGCCAATATGAACAAAG NGG 2 0.45 Tier II
6 NC_000013.11 13 46556293 + LRCH1 NNNAGCCCATCTGAACAGAG NGG 2 0.36 Tier II
7 NC_000002.12 2 153680719 - GALNT13 NNNGGGCCATATGGACAGAG NGG 2 0.2333 Tier II
8 NC_000015.10 15 25859479 + ATP10A NNNGGTCCATATGATCAGAG NGG 2 0.1857 Tier II
9 NC_000004.12 4 147800368 + ARHGAP10 NNNGGCCCATGTGACCAGAG NGG 2 0.1773 Tier II
10 NC_000015.10 15 43257441 + TGM5 NNNTGCCCATATGAACAGAG NGT 2 0.0059 Tier II
11 NC_000011.10 11 72016559 + LOC100128494 NNNGACCCATGTGAACAGAG NGG 2 0.5633 Tier III
12 NC_000023.11 X 39798234 + LOC105373177 NNNGGACCATAGGAACAGAG NGG 2 0.4643 Tier III
13 NC_000002.12 2 95007286 - MAL-AS1 NNNGGCCAATCTGAACAGAG NGG 2 0.26 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 97483425 + Dcn NNNGGACCATTTGAACAGAG NGG 2 0.2857 Tier I
2 NC_000085.6 19 30857565 - Prkg1 NNNGACCCAAATGAACAGAG NGG 2 0.7429 Tier II
3 NC_000072.6 6 142147523 - Slco1a6 NNNGGCCAATATGAACAGAG NGG 1 0.65 Tier II
4 NC_000081.6 15 95408414 - Nell2 NNNGGCCTATATGAACATAG NGG 2 0.5833 Tier II
5 NC_000068.7 2 41603321 - Lrp1b NNNGGCCAATATGAACATAG NGG 2 0.4333 Tier II
6 NC_000070.6 4 66210165 + Astn2 NNNGGCCAATATGAACATAG NGG 2 0.4333 Tier II
7 NC_000070.6 4 111277782 - Agbl4 NNNGGCCAATATGAACATAG NGG 2 0.4333 Tier II
8 NC_000076.6 10 79526564 + Plpp2 NNNGGTCCATATGGACAGAG NGG 2 0.4333 Tier II
9 NC_000072.6 6 80533928 + Lrrtm4 NNNGGCCCCTATGAACATAG NGG 2 0.381 Tier II
10 NC_000086.7 X 129902473 + Diaph2 NNNGGCCCCTATGAACATAG NGG 2 0.381 Tier II
11 NC_000079.6 13 13733320 + Lyst NNNGGCCACTATGAACAGAG NGG 2 0.3714 Tier II
12 NC_000082.6 16 81232105 + Ncam2 NNNGGCCACTATGAACAGAG NGG 2 0.3714 Tier II
13 NC_000084.6 18 7385623 - Mpp7 NNNGGCCACTATGAACAGAG NGG 2 0.3714 Tier II
14 NC_000075.6 9 100883966 + Stag1 NNNGGCCGCTATGAACAGAG NGG 2 0.3673 Tier II
15 NC_000070.6 4 155290081 - Prkcz NNNGGCCCATGTGTACAGAG NGG 2 0.3467 Tier II
16 NC_000073.6 7 140255865 - 5830411N06Rik NNNGGCCAATATGAACACAG NGG 2 0.3095 Tier II
17 NC_000077.6 11 43570330 + Ccnjl NNNGGCCCATAGGAACAGTG NGG 2 0.2692 Tier II
18 NC_000077.6 11 103840500 - Nsf NNNGCCCCATATGAACAGAG NAG 2 0.2037 Tier II
19 NC_000075.6 9 68716609 - Rora NNNTGCCCATATGTACAGAG NGG 2 0.1939 Tier II
20 NC_000079.6 13 51458878 - Shc3 NNNGGCCCATATGAACAGAG NTA 2 0.0 Tier II
21 NC_000069.6 3 159205101 - Gm20752 NNNGGCTCCTATGAACAGAG NGG 2 0.4643 Tier III
22 NC_000067.6 1 79024393 - Gm29536 NNNGGCCCCTATGAACATAG NGG 2 0.381 Tier III
23 NC_000071.6 5 28641061 + 9530036O11Rik NNNTGCCAATATGAACAGAG NGG 2 0.2364 Tier III
24 NC_000074.6 8 92003997 - Gm36843 NNNGCCCCATATTAACAGAG NGG 2 0.2357 Tier III
Download CSV

Other clones with same target sequence:

(none)