Construct: sgRNA BRDN0001145937

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGTGCCATAGCCAGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST1 (22983)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76122

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 12843568 - MAST1 NNNTGCCATAGCCAGATGAA NGG 0 1.0 Tier I
2 NC_000005.10 5 67095636 - MAST4 NNNTCCCATAGCCAGAGGAA NGG 2 0.0924 Tier I
3 NC_000012.12 12 125003051 - BRI3BP NNNTGCAAAAGCCAGATGAA NGG 2 0.6964 Tier II
4 NC_000003.12 3 89169920 + EPHA3 NNNTGACAGAGCCAGATGAA NGG 2 0.5748 Tier II
5 NC_000005.10 5 156704529 + SGCD NNNTGCTATAGCTAGATGAA NGG 2 0.5688 Tier II
6 NC_000008.11 8 98008755 + MATN2 NNNTGCCAAAGCCAGATGTA NGG 2 0.5 Tier II
7 NC_000009.12 9 132988683 + GFI1B NNNTGCCAGAGTCAGATGAA NGG 2 0.3333 Tier II
8 NC_000006.12 6 38170108 + BTBD9 NNNAGCCAGAGCCAGATGAA NGG 2 0.2948 Tier II
9 NC_000009.12 9 72538945 - TMC1 NNNTGCCATAGCAAGATGAG NGG 2 0.2941 Tier II
10 NC_000009.12 9 18608720 - ADAMTSL1 NNNTTCCAGAGCCAGATGAA NGG 2 0.1857 Tier II
11 NC_000005.10 5 14821758 - ANKH NNNTGCCATAGCCACATGGA NGG 2 0.1023 Tier II
12 NC_000002.12 2 177884425 + PDE11A NNNTGCAATAGCCAGATGAA NTG 2 0.0292 Tier II
13 NC_000006.12 6 2348456 - GMDS-DT NNNTGACAAAGCCAGATGAA NGG 2 0.8622 Tier III
14 NC_000005.10 5 156704529 + LOC105377673 NNNTGCTATAGCTAGATGAA NGG 2 0.5688 Tier III
15 NC_000008.11 8 95347777 + CFAP418-AS1 NNNTGACTTAGCCAGATGAA NGG 2 0.398 Tier III
16 NC_000006.12 6 163904592 + LOC105378102 NNNTGCCATGCCCAGATGAA NGG 2 0.2381 Tier III
17 NC_000006.12 6 145754594 + EPM2A-DT NNNTGCCATAGCCAGAGGGA NGG 2 0.0441 Tier III
18 NC_000006.12 6 145754594 + LOC105378039 NNNTGCCATAGCCAGAGGGA NGG 2 0.0441 Tier III
19 NC_000014.9 14 56909020 - OTX2-AS1 NNNTGCCATAGCCATATGAA NGC 2 0.0032 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 84930860 + Mast1 NNNTGCCATAGCCAGAGGAA NGG 1 0.1176 Tier I
2 NC_000081.6 15 91311032 + Slc2a13 NNNTGTCATAGCCAGATGAT NGG 2 0.5571 Tier II
3 NC_000081.6 15 92746841 + Pdzrn4 NNNTGCCAAGGCCAGATGAA NGG 2 0.5159 Tier II
4 NC_000073.6 7 87726370 + Grm5 NNNTGTCATAGTCAGATGAA NGG 2 0.5 Tier II
5 NC_000072.6 6 85010975 + Exoc6b NNNTGGCAGAGCCAGATGAA NGG 2 0.3095 Tier II
6 NC_000083.6 17 56414235 - Ptprs NNNTGGCATAGTCAGATGAA NGG 2 0.2692 Tier II
7 NC_000086.7 X 129875378 + Diaph2 NNNTGCCATATCCAGAAGAA NGG 2 0.2051 Tier II
8 NC_000086.7 X 143531514 + Pak3 NNNTGCCTTAGCAAGATGAA NGG 2 0.1648 Tier II
9 NC_000070.6 4 135370144 - Ncmap NNNTCCCATAGCCAGATGCA NGG 2 0.1626 Tier II
10 NC_000071.6 5 102394198 - Arhgap24 NNNTGTCATAGCCAGAGGAA NGG 2 0.1092 Tier II
11 NC_000071.6 5 23588634 + Srpk2 NNNTGCCTTAGCCAGATGCA NGG 2 0.0887 Tier II
12 NC_000074.6 8 46599256 - Primpol NNNTGCCATAGACAGATGAA NGC 2 0.0159 Tier II
13 NC_000078.6 12 89589282 + Nrxn3 NNNTGGCATAGCCAGATGAA NGC 2 0.0111 Tier II
14 NC_000083.6 17 86419469 + Prkce NNNTGACATAGCCAGCTGAA NGG 2 0.0 Tier II
15 NC_000069.6 3 153464452 - St6galnac3 NNNTGCCATAGCCAGATGAA NCA 2 0.0 Tier II
16 NC_000071.6 5 16321080 - Cacna2d1 NNNTGCCATAGCCATCTGAA NGG 2 0.0 Tier II
17 NC_000072.6 6 94352884 + Gm19908 NNNGGCCATAGCCAGATGAA NAG 2 0.162 Tier III
18 NC_000073.6 7 78632390 + E430016F16Rik NNNTTCCATAGCCAGGTGAA NGG 2 0.0577 Tier III
19 NC_000074.6 8 46599256 - Gm45607 NNNTGCCATAGACAGATGAA NGC 2 0.0159 Tier III
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Other clones with same target sequence:

(none)