Construct: sgRNA BRDN0001145937
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTGCCATAGCCAGATGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST1 (22983)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76122
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000019.10 |
19 |
12843568 |
- |
MAST1 |
NNNTGCCATAGCCAGATGAA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000005.10 |
5 |
67095636 |
- |
MAST4 |
NNNTCCCATAGCCAGAGGAA |
NGG |
2 |
0.0924 |
Tier I |
3 |
NC_000012.12 |
12 |
125003051 |
- |
BRI3BP |
NNNTGCAAAAGCCAGATGAA |
NGG |
2 |
0.6964 |
Tier II |
4 |
NC_000003.12 |
3 |
89169920 |
+ |
EPHA3 |
NNNTGACAGAGCCAGATGAA |
NGG |
2 |
0.5748 |
Tier II |
5 |
NC_000005.10 |
5 |
156704529 |
+ |
SGCD |
NNNTGCTATAGCTAGATGAA |
NGG |
2 |
0.5688 |
Tier II |
6 |
NC_000008.11 |
8 |
98008755 |
+ |
MATN2 |
NNNTGCCAAAGCCAGATGTA |
NGG |
2 |
0.5 |
Tier II |
7 |
NC_000009.12 |
9 |
132988683 |
+ |
GFI1B |
NNNTGCCAGAGTCAGATGAA |
NGG |
2 |
0.3333 |
Tier II |
8 |
NC_000006.12 |
6 |
38170108 |
+ |
BTBD9 |
NNNAGCCAGAGCCAGATGAA |
NGG |
2 |
0.2948 |
Tier II |
9 |
NC_000009.12 |
9 |
72538945 |
- |
TMC1 |
NNNTGCCATAGCAAGATGAG |
NGG |
2 |
0.2941 |
Tier II |
10 |
NC_000009.12 |
9 |
18608720 |
- |
ADAMTSL1 |
NNNTTCCAGAGCCAGATGAA |
NGG |
2 |
0.1857 |
Tier II |
11 |
NC_000005.10 |
5 |
14821758 |
- |
ANKH |
NNNTGCCATAGCCACATGGA |
NGG |
2 |
0.1023 |
Tier II |
12 |
NC_000002.12 |
2 |
177884425 |
+ |
PDE11A |
NNNTGCAATAGCCAGATGAA |
NTG |
2 |
0.0292 |
Tier II |
13 |
NC_000006.12 |
6 |
2348456 |
- |
GMDS-DT |
NNNTGACAAAGCCAGATGAA |
NGG |
2 |
0.8622 |
Tier III |
14 |
NC_000005.10 |
5 |
156704529 |
+ |
LOC105377673 |
NNNTGCTATAGCTAGATGAA |
NGG |
2 |
0.5688 |
Tier III |
15 |
NC_000008.11 |
8 |
95347777 |
+ |
CFAP418-AS1 |
NNNTGACTTAGCCAGATGAA |
NGG |
2 |
0.398 |
Tier III |
16 |
NC_000006.12 |
6 |
163904592 |
+ |
LOC105378102 |
NNNTGCCATGCCCAGATGAA |
NGG |
2 |
0.2381 |
Tier III |
17 |
NC_000006.12 |
6 |
145754594 |
+ |
EPM2A-DT |
NNNTGCCATAGCCAGAGGGA |
NGG |
2 |
0.0441 |
Tier III |
18 |
NC_000006.12 |
6 |
145754594 |
+ |
LOC105378039 |
NNNTGCCATAGCCAGAGGGA |
NGG |
2 |
0.0441 |
Tier III |
19 |
NC_000014.9 |
14 |
56909020 |
- |
OTX2-AS1 |
NNNTGCCATAGCCATATGAA |
NGC |
2 |
0.0032 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000074.6 |
8 |
84930860 |
+ |
Mast1 |
NNNTGCCATAGCCAGAGGAA |
NGG |
1 |
0.1176 |
Tier I |
2 |
NC_000081.6 |
15 |
91311032 |
+ |
Slc2a13 |
NNNTGTCATAGCCAGATGAT |
NGG |
2 |
0.5571 |
Tier II |
3 |
NC_000081.6 |
15 |
92746841 |
+ |
Pdzrn4 |
NNNTGCCAAGGCCAGATGAA |
NGG |
2 |
0.5159 |
Tier II |
4 |
NC_000073.6 |
7 |
87726370 |
+ |
Grm5 |
NNNTGTCATAGTCAGATGAA |
NGG |
2 |
0.5 |
Tier II |
5 |
NC_000072.6 |
6 |
85010975 |
+ |
Exoc6b |
NNNTGGCAGAGCCAGATGAA |
NGG |
2 |
0.3095 |
Tier II |
6 |
NC_000083.6 |
17 |
56414235 |
- |
Ptprs |
NNNTGGCATAGTCAGATGAA |
NGG |
2 |
0.2692 |
Tier II |
7 |
NC_000086.7 |
X |
129875378 |
+ |
Diaph2 |
NNNTGCCATATCCAGAAGAA |
NGG |
2 |
0.2051 |
Tier II |
8 |
NC_000086.7 |
X |
143531514 |
+ |
Pak3 |
NNNTGCCTTAGCAAGATGAA |
NGG |
2 |
0.1648 |
Tier II |
9 |
NC_000070.6 |
4 |
135370144 |
- |
Ncmap |
NNNTCCCATAGCCAGATGCA |
NGG |
2 |
0.1626 |
Tier II |
10 |
NC_000071.6 |
5 |
102394198 |
- |
Arhgap24 |
NNNTGTCATAGCCAGAGGAA |
NGG |
2 |
0.1092 |
Tier II |
11 |
NC_000071.6 |
5 |
23588634 |
+ |
Srpk2 |
NNNTGCCTTAGCCAGATGCA |
NGG |
2 |
0.0887 |
Tier II |
12 |
NC_000074.6 |
8 |
46599256 |
- |
Primpol |
NNNTGCCATAGACAGATGAA |
NGC |
2 |
0.0159 |
Tier II |
13 |
NC_000078.6 |
12 |
89589282 |
+ |
Nrxn3 |
NNNTGGCATAGCCAGATGAA |
NGC |
2 |
0.0111 |
Tier II |
14 |
NC_000083.6 |
17 |
86419469 |
+ |
Prkce |
NNNTGACATAGCCAGCTGAA |
NGG |
2 |
0.0 |
Tier II |
15 |
NC_000069.6 |
3 |
153464452 |
- |
St6galnac3 |
NNNTGCCATAGCCAGATGAA |
NCA |
2 |
0.0 |
Tier II |
16 |
NC_000071.6 |
5 |
16321080 |
- |
Cacna2d1 |
NNNTGCCATAGCCATCTGAA |
NGG |
2 |
0.0 |
Tier II |
17 |
NC_000072.6 |
6 |
94352884 |
+ |
Gm19908 |
NNNGGCCATAGCCAGATGAA |
NAG |
2 |
0.162 |
Tier III |
18 |
NC_000073.6 |
7 |
78632390 |
+ |
E430016F16Rik |
NNNTTCCATAGCCAGGTGAA |
NGG |
2 |
0.0577 |
Tier III |
19 |
NC_000074.6 |
8 |
46599256 |
- |
Gm45607 |
NNNTGCCATAGACAGATGAA |
NGC |
2 |
0.0159 |
Tier III |
Other clones with same target sequence:
(none)