Construct: sgRNA BRDN0001145943
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGACAACATCGAAGTAGCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRL (5157)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77697
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
17628519 |
- |
PDGFRL |
NNNACAACATCGAAGTAGCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
31674470 |
- |
OTUD7A |
NNNACAACATCTAAGAAGCT |
NGG |
2 |
0.3497 |
Tier II |
| 3 |
NC_000004.12 |
4 |
21422630 |
+ |
KCNIP4 |
NNNACAACATTGAAGTAGTT |
NGG |
2 |
0.142 |
Tier II |
| 4 |
NC_000005.10 |
5 |
172987877 |
- |
ATP6V0E1 |
NNNACAACAACGAATTAGCT |
NGG |
2 |
0.1224 |
Tier II |
| 5 |
NC_000011.10 |
11 |
8975038 |
+ |
TMEM9B-AS1 |
NNNACAACATCGGAGTATCT |
NGG |
2 |
0.4348 |
Tier III |
| 6 |
NC_000015.10 |
15 |
30731291 |
+ |
LOC100288637 |
NNNACAACATCTAAGAAGCT |
NGG |
2 |
0.3497 |
Tier III |
| 7 |
NC_000011.10 |
11 |
78141292 |
- |
KCTD21-AS1 |
NNNAAAACATCTAAGTAGCT |
NGG |
2 |
0.2198 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
40985566 |
- |
Pdgfrl |
NNNACAACATCAAAGTAACT |
NGG |
2 |
0.6462 |
Tier I |
| 2 |
NC_000080.6 |
14 |
122741113 |
+ |
Pcca |
NNNAGAACATAGAAGTAGCT |
NGG |
2 |
0.45 |
Tier II |
| 3 |
NC_000086.7 |
X |
142321761 |
+ |
Acsl4 |
NNNAGAACATAGAAGTAGCT |
NGG |
2 |
0.45 |
Tier II |
| 4 |
NC_000071.6 |
5 |
129601886 |
- |
Mmp17 |
NNNAGAACATCGAAGCAGCT |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000071.6 |
5 |
24156495 |
- |
Klhl7 |
NNNACAACATCGAAGTATCA |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000076.6 |
10 |
9383978 |
- |
Samd5 |
NNNAAAACTTCGAAGTAGCT |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000084.6 |
18 |
80985280 |
+ |
Sall3 |
NNNACAACCTCGAACTAGCT |
NGG |
2 |
0.1558 |
Tier II |
| 8 |
NC_000076.6 |
10 |
5171441 |
+ |
Syne1 |
NNNACAACATCTAAGGAGCT |
NGG |
2 |
0.1331 |
Tier II |
| 9 |
NC_000072.6 |
6 |
32257056 |
- |
Plxna4 |
NNNACAACATCGAAGTACCT |
NAG |
2 |
0.1235 |
Tier II |
| 10 |
NC_000085.6 |
19 |
47626019 |
- |
Slk |
NNNACAACATGGAAGTAGCT |
NAG |
2 |
0.0648 |
Tier II |
Other clones with same target sequence:
(none)
